甲基强的松龙对脊髓损伤后卵磷脂胆固醇脂酰转移酶基因表达的影响  

Effect of high dose methylprednisolone on lecithin-cholesterolacyltransferase mRNA expression after spinal cord injury

作　　者：徐杰[1] 罗奋棋[1] 吴世强[1] 

机构地区：[1]福建省立医院骨二科,350001

出　　处：《福建医药杂志》2008年第5期1-2,共2页Fujian Medical Journal

基　　金：福建省自然科学基金项目(2006J0327);福建省科技厅青年人才项目(2004J071)

摘　　要：目的研究甲基强的松龙对脊髓损伤后卵磷脂胆固醇脂酰转移酶(LCAT)基因表达的影响。方法SD大鼠30只,体重200～250 g,雌雄不拘。随机分为假手术组、单纯脊髓损伤组(损伤组)、脊髓损伤+大剂量甲基强的松龙组(甲基强的松龙组)。每组10只。损伤后24小时将损伤平面上下0.5 cm的脊髓组织切取,进行RT-PCR反应。结果假手术组在术后24小时LCAT mRNA的表达的相对丰度为0.6743±0.0043,损伤组在伤后24小时LCAT mRNA的表达相对丰度降至0.0589±0.0281,差异有统计学意义(P<0.01)。甲基强的松龙组在伤后24小时LCAT mRNA的表达的相对丰度为0.7036±0.2225,与损伤组比较差异有统计学意义(P<0.01)。结论大剂量甲基强的松龙能促进LCAT的表达,发挥保护作用,减少脊髓继发损伤。Objective To investigate effect of high dose methylprednisolone （MP） on lecithin-cholesterolacyltransferase mRNA expression after spinal cord injury. Methods Thirty SD rats were divided equally into operation but none injury group, Allen＇s weight drop injury group and injury with high dose MP group. Twenty four hours after the operation, the spinal lesion and neighbor areas were moved for examination of lecithin-cholesterolaeyltransferase mRNA expression by Retrograde Polymerase Chain Reaction technique （RT PCR）. Results The lecithin-cholesterolacyltransferase mRNA expression were 0. 6743 ± 0. 0043, 0. 0589±0. 0281 （P〈0.01）, 0. 7036±0. 2225 （P〈0. 01） in three group. It increased significantly after spinal cord injury and treatment with MP. Conclusion MP can greatly increase the expression of lecithin-cholesterolacyltransferase and protect the neighbor areas after spinal cord injury.

关 键 词：脊髓损伤 卵磷脂胆固醇脂酰转移酶 基因表达 RT-PCR技术 

分 类 号：R651.2[医药卫生—外科学]