高效液相色谱法测定金银花提取物中绿原酸的含量  

Determination of Chlorogenic Acid in the Extract of Flos Lonicerae by HPLC

作　　者：刘磊[1] 何再安[1] 余子川[1] 刘超英[1] 刘焱文[1] 

机构地区：[1]湖北中医学院.中药资源与中药复方省部共建教育部重点实验室,湖北武汉430061

出　　处：《时珍国医国药》2008年第10期2416-2417,共2页Lishizhen Medicine and Materia Medica Research

摘　　要：目的建立金银花提取物中绿原酸含量的HPLC测定方法。方法国产C18柱（4．6mm×250mm，7μm）；流动相为乙腈：0．4％磷酸（12：88）；检测波长为327nm；流速1ml·min^-1；选样量10μl；柱温25％。结果绿原酸在0．0716～0．6444μg范围内线性关系良好，r=0．9996；平均回收率99．99％，RSD=1．61％（n=6）。结论该方法简便、快速、准确，可用于金银花提取物的含量测定及质量控制。Objective To establish a method for the determination of Chlorogenic acid in the extract of Flos Lonicerae. Methods The chrmnatographic conditions were as follows： C18 chromatographic column （4.6 mm× 250 mm,7 μm）, a mobile phase of acetonitrile and 0.4% H3PO4 （12：88） ,the detection wavelength at 327 nm and the flow rate being 1 ml · min^-1. Resuits A linearity of Chlorogenie acid in the extract was obtained in the range of 0. 071 6 -0. 644 4 μg r = 0.999 6 （n = 6）. The average recovery was 99.99% and RSD = 1.61% （ n = 6 ）. Conclusion This method is easy, sensitive and accurate for the determination of Chlorogenic acid in the extract of Flos Lonicerae.

关 键 词：金银花提取物 绿原酸 高效液相色谱法 

分 类 号：R284.2[医药卫生—中药学]