多重耐药鲍曼不动杆菌消毒剂耐药基因检测及同源性分析  

Detection and analysis of the homogeneity of disinfectants resistant gene in multi-drug resistant Acinetobacter baumannii

作　　者：侯天文[1] 陈兴[1] 李玮[1] 陈晶[1] 白云[1] 徐铮[1] 

机构地区：[1]白求恩国际和平医院检验实验中心,河北石家庄050082

出　　处：《中国微生态学杂志》2008年第5期475-477,共3页Chinese Journal of Microecology

基　　金：河北省科技攻关计划项目(04276184)

摘　　要：目的探讨多重耐药鲍曼不动杆菌(MDRAB)临床分离株对医院常用消毒剂苯扎溴铵和醋酸氯已定的耐药表型与qacE△1-sul1基因型的相关性并分析菌株的同源性。方法用微量肉汤稀释法检测菌株的最低抑菌浓度(MIC)和最低杀菌浓度(MBC),用PCR方法检测qacE△1-sul1基因,用脉冲场凝胶电泳(PFGE)分析菌株的同源性。结果20株MDRAB中,18株(90%)qacE△1-sul1阳性,2株(10%)qacE△1-sul1阴性。qacE△1-sul1阳性株对苯扎溴铵的MIC50、MIC90、MBC50和MBC90分别是qacE△1-sul1阴性株的2倍、2倍、8倍和8倍。而qacE△1-sul1阳性株对醋酸氯已定的MIC50、MIC90、MBC50和MBC90分别是qacE△1-sul1阴性株的2倍、2倍、4倍和8倍。PF-GE显示:18株qacE△1-sul1阳性MDRAB可分为A^F6个PFGE克隆。2株qacE△1-sul1阴性MDRAB均为B克隆。结论MDRAB对消毒剂耐药性升高与qacE△1-sul1基因相关,临床存在多个克隆株的传播。Objective To investigate the resistance to benzalkonium bromide and chlorhexidine acetate and disin- fectants-resistant gene of qacE△1-sul1 in clinical isolates of multi-drug resistant Acinetobacter baumannii, and to analyse the Homogeneity of these strains. Methods The minimal inhibitory concentration （M1C） and minimal bactericide concentration of disinfectants were determined by the micro-dilution method, the gene of qacE △ 1-sul1 were analyzed using PCR, the organism typing was performed by pulsed-field gel electrophoresis （PFGE）. Results Among the 20 isolates of multi-drug resistant Acinetobacter baumannii, 18 were positive for qacE△1-sul1 gene, 2 were negative. The MIC50, MIC90, MBC50 and MBC90 of benzalkonium bromide for qacE△1-sul1 gene positive isolates were 2-fold,2-fold,8-fold and 8-fold to qacE△1 gene negative isolates, while the MIC500, MIC90, MBC50 and MBC90 of chlorhexidine acetate for qacE△1-sul1 gene positive isolates were 2-fold,2-fold,4-fold and 8-fold to qacE△1 gene negative isolates. 18 isolates for qacE△1-sul1 gene positive belonged to 6 different PFGE typing （A-F） ,the other two isolates for qacE△1-sul1 gene negative belonged to B typing of PFGE. Conclusions The increase of resistance multi-drug resistant Acinetobacter baumannii isolates against disinfectant was related to the qacE△1-sul1 genes in them, and there were multiple different PFGE typing of multi-drug resistant Acine-tobacter baumannii existing in clinical wards.

关 键 词：鲍曼不动杆菌 消毒剂 多重耐药 基因 脉冲场凝胶电泳 

分 类 号：R378[医药卫生—病原生物学]