不同栀子饮片二萜色素类成分比较研究  

Content comparison of diterpenoid pigments in defferent processed products of Gardenia jasminoides

作　　者：张村[1] 肖永庆[1] 李丽[1] 李桂柳[1] 逄镇[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2008年第21期2470-2473,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30672666)

摘　　要：目的:对栀子不同饮片二萜色素类成分进行比较研究。方法:HPLC同时测定4个主要成分(crocin1,crocin 2,crocin 3,crocetin)含量,Kromasil C18柱,流动相为甲醇-乙腈-0.3%甲酸水不同梯度洗脱,检测波长440nm,流速1.0 mL.min-1,柱温35℃。结果:4个成分在测定范围内线性关系较好(r>0.999 5),平均回收率分别为97.77%,100.05%,98.40%,101.02%。结论:所建立的方法准确、可靠。栀子炮制前后色素类成分发生了明显变化,栀子不同饮片的含量变化呈现一定的规律性。Objective ： To compare the contents of diterpenoid pigments in the different processed products of Gardenia jctsminoides. Method： The separation of Crocin 1 , Crocin 2, Croein 3, Croeetin were determined simultaneously by HPLC on a kromasil C18 column at 35 ℃ with the m methanol-aeetonitrile-0. 3% formic acid anhydrous in gradient elution as the mobile phrase. The detection wavelength was set at 440 nm and the flow rate was 1.0 mL · min^-1. Result： The obtained linearity of the four components was better over 0. 999 5 and the average recoveries were 97.77% , 100. 05% , 98.40% , 101.02% , respectively. Conclusion： The method is simple, accurate with good reproducibility. The results showed that the remarkable variation regulations appear among the different processed products.

关 键 词：栀子饮片 二萜色素 HPLC 含量测定 

分 类 号：R284.1[医药卫生—中药学]