聚酰胺分离纯化喜树果中10-羟基喜树碱和喜果苷的研究  

Isolation and purification of 10-hydroxycamptothecin and vincoside-lactam from Camptotheca acuminata seed by polyamide

作　　者：史伟国[1,2] 祖元刚[1,2] 杨磊[1,2] 赵春建[1,2] 李佳慧 

机构地区：[1]东北林业大学植物药工程研究中心,黑龙江哈尔滨150040 [2]东北林业大学森林植物生态学教育部重点实验室,黑龙江哈尔滨150040

出　　处：《中国中药杂志》2008年第21期2486-2489,共4页China Journal of Chinese Materia Medica

基　　金：国家科技支撑计划项目(2006BAD18B04)

摘　　要：目的:研究聚酰胺分离纯化10-羟基喜树碱和喜果苷的工艺条件与参数。方法:采用HPLC进行定量分析,考察了聚酰胺对10-羟基喜树碱和喜果苷静态和动态吸附性能,确定最佳的工艺条件。结果:优化的工艺条件为:药液中10-羟基喜树碱和喜果苷的质量浓度分别为0.189,0.334 g.L-1,上样液pH6,上样流速1.0 mL.min-1,上样量3 BV,洗脱剂60%乙醇溶液,洗脱流速1.0 mL.min-1,洗脱剂用量5 BV。经聚酰胺分离纯化后10-羟基喜树碱和喜果苷的质量分数分别为17.52%,32.87%,收率分别为66.05%,75.86%。结论:聚酰胺对10-羟基喜树碱和喜果苷吸附能力强,解吸容易,分离效率果较好,此工艺为大规模产业化提供了依据。To investigate the technological parameters of the isolation and purification of 10-hydroxycamptothecin and vincoside-lactam from Camptotheca acuminata seed by polyamide. The static and dynamic adsorption characteristics of 10-hydroxycamptothecin and vincoside-lactam on polyamide were studied, and the contents were determined by HPLC. The optimum parameters for adsorption were as follows： the contents of 10-hydroxycmnptothecin and vincoside-lactam in the extracts were 0. 189 g·L^-1and 0. 334 g·L^-1, respectively, pH 6, flow rate was 1.0 mL · min^-1, processing volume was 3 BV; for desorption： ethanol-water （60： 40）, flow rate was 1.0 mL · min^-1, 5 BV as an eluent. After treated with polyamide, the contents of 10-hydroxycamptothecin and vincoside-lactam were 17.52% and 32. 87% , respectively, the recovery yields were 66. 05% and 75.86%, respectively. Results showed that poly- amide revealed a good ability to separate 10-hydroxycamptothecin and vincoside-lactam. Therefore, we concluded that results in this study may provide scientific references for the large-scale production of 10-hydroxycamptothecin and vincoside-lactam extracted from C. acuminata seed.

关 键 词：10-羟基喜树碱 喜果苷 喜树果 聚酰胺 

分 类 号：R284.2[医药卫生—中药学]