胡桃提取物抗HIV-1病毒的初步研究  

Investigation on inhibition of HIVⅢB virus with extractions of Juglans regia

作　　者：刘兆梅[1] 温瑞兴[1] 马洪涛[1] 杨怡姝[1] 王小利[1] 吕岫华[1] 李泽琳[1] 

机构地区：[1]北京工业大学生命科学与工程学院,北京100022

出　　处：《中国中药杂志》2008年第21期2535-2538,共4页China Journal of Chinese Materia Medica

基　　金：北京市自然科学基金预探索项目(7073087)

摘　　要：目的:研究胡桃提取物抗HIV-1病毒的作用,寻找新型高效的抗HIV病毒中药先导化合物。方法:采用植物化学柱层析等技术对胡桃进行提取分离,通过体外药效学实验,观察胡桃提取物的抗HIV-1病毒作用,并结合作用靶点的研究对胡桃提取物的作用机制进行探讨。结果:胡桃提取物具有很好的抗HIV-1作用,筛选出6个抗艾滋病毒作用显著的有效部位,其中2个有效部位的作用靶点分别为gp-41跨膜蛋白和HIV整合酶。结论:胡桃提取物具有很强的体外抗HIV-1病毒作用。Objective： To study the effect of anti-HIV Ⅲ B virus with extractions from Juglans regia, so as to searching for the new and efficacious leading compound of AIDS therapy. Method： Phytochemical and chromatographical techniques were used to isolate compounds from J. regia;MT4 cells and HIVⅢB virus were used to study the effect of anti-HIV activity in vitro. BIACORE^3000 molecule coupled equipment were used for the target research also. Result： Two extractions （B＆E） were isolated from J. regia which possess the effect of anti-HIV activity. Targets study found that extraction B could affected on HIV-1 gp-41 fusing protein and extraction E could affected on HIV-1 integrase respectively. Conclusion： J. regia is a traditional Chinese medicine with active anti-HIV activity and worth to be studied further.

关 键 词：胡桃提取物 提取分离 抗HIV-1病毒 作用靶点 

分 类 号：R285.5[医药卫生—中药学]