高氡地区人群GSTT1基因多态性与肺癌易感关系的初步研究  

A primary case-control study on the relationship between genetic polymorphisms of GSTT1 and lung cancer susceptibility to the people living in high radon-exposed area

作　　者：齐雪松[1] 吕慧敏[1] 夏英[1] 尚兵[1] 孙全富[1] 崔宏星[1] 王利平[1] 

机构地区：[1]中国疾病预防控制中心辐射防护与核安全医学所,北京100088

出　　处：《中国职业医学》2008年第5期361-363,367,共4页China Occupational Medicine

基　　金：国家自然科学基金资助项目(3047053039970235)

摘　　要：目的研究高氡暴露地区人群中GSTT1基因多态性与肺癌易感性的关系。方法采用病例一对照研究方法，以多重聚合酶链反应扩增（Multiplex—PCR）技术，对高氡暴露地区53例肺癌患者和72例对照人员进行了代谢酶GSTT1基因多态性检测，并分析了不同人群中该基因多态性与肺癌发病风险的关系。结果GSTT1基因功能型和缺陷型在肺癌组分布频率分别是67．9％、32．1％，在对照组为62．5％、37．5％。GSTT1基因多态性与肺癌发病风险无显著关联，但考虑到氡暴露因素后GSTT1基因缺陷型的肺癌发病风险有所增高[有效剂量≥50mSv时OR值1．49，95％可信限（CI）0．52～4．20；有效剂量〈50mSv时OR值1．14，95％CI 0．20～6．60]，年龄在加～59岁人群中GSTT1基因缺陷型的肺癌发病风险增至1．81倍（95％CI 0．67～4．86）。同时携带GSTT1缺陷型和GSTM1基因功能型的肺癌发病风险是GSTM1基因功能型和GSTT1基因功能型的1．20倍（95％CI 0．36—4．00），同时携带GSTT1基因功能型和GSTM1基因缺陷型的肺癌发病风险是GSTM1基因功能型和GSTT1基因功能型的1．82倍（95％CI 0．73～4．58）。结论GSTT1基因多态性在肺癌人群中的分布频率与在对照人群中的分布频率差异无显著性（P〉0．05）。GSTT1基因和GSTM1基因联合多态性比单基因多态性对肺癌发病风险增高更明显，但差异无统计学意义。Objective To assess the role of genetic polymorphisms of metabolizing enzymes Glutathione S-transferases T1 （ GSTT1 ） in the risk of developing lung cancer to the people living in high radon-exposed area. Methods A case-control study was performed with 53 lung cancer patients and 72 frequency-matched controls. The associations between the genotypes and risk of developing lung cancer were estimated by odds ratios （OR） and their 95% confidence intervals （CI） calculated by unconditional logistic regression. Results The frequencies of GSTT1 positive polymorphism and null polymorphism were 67. 9% and 32. 1% respectively in lung cancer patients. While 62. 5% and 37.5% respectively in the controls. No statistical significant association was found between GSTT1 null polymorphism and lung cancer. The GSTT1 null polymorphism in the people with high radon exposure could increase the risk of developing lung cancer （ ≥50 mSv, OR 1.49, 95% CI 0. 52 -4. 20, 〈50 mSv, OR 1.14, 95% CI 0. 20 -6. 60）. In the case of the people carrying GSTT1 null polymorphism aged between 40 and 59, the risk was 1.81-fold higher than that of people carrying GSTT1 functional polymorphism. Combined analysis showed that the risk of de- veloping lung cancer for the people carting both GSTT1 null and GSTM1 positive was higher（OR 1.20, 95% CIO. 36 -4. 00）. The risk of GSTT1 positive and GSTM1 null combined was 1.82 （95% CI 0. 73 - 4.58）. Conclusion The frequency of CSTT1 polymorphism in lung cancer patients has no statistical significant difference compared with that of the controls. GSTM1 and GSTT1 might have a tendency of increasing the risk of developing lung cancer, but the difference was not statistically significant.

关 键 词：氡 GSTT1 基因多态性 肺癌易感性 

分 类 号：X591[环境科学与工程—环境工程]