苯醌致HL-60细胞增殖方法的研究  

Establishment of a method for enhancement of myeloid cell growth induced by benzoquinone

作　　者：李永胜[1] 肖勇梅[1] 刘移民[1,2] 李旭东[1] 郭晓[1] 张维森 

机构地区：[1]中山大学公共卫生学院,广东广州510800 [2]广州市职业病防治院,510620

出　　处：《中国职业医学》2008年第5期382-384,共3页China Occupational Medicine

基　　金：广州市科技攻关项目(2005Z3-E0131);广州市医疗卫生机构重点专科重点项目(2004Z011)

摘　　要：目的建立引起该细胞增殖的方法,为以后进一步研究染毒后细胞内结构与功能的变化提供方法基础。方法用苯醌对人早幼细胞系HL-60进行染毒,取对数生长期的HL-60细胞,用不同浓度的苯醌进行染毒,行台盼蓝拒染法测细胞生存率,Alamar blue法测细胞增殖率,流式细胞技术测细胞周期分布。结果苯醌浓度为3μmol/L的剂量组HL-60细胞存活率无明显改变,且增殖率最高,细胞进入S期的比例[(48.23±1.37)%]高于对照组[(42.47±0.45)%]。结论用3μmol/L的苯醌染毒HL-60细胞,可引起该细胞明显增殖。Objective To establish a method for proliferation of HL-60 cells for further research on the structural and func- tional changes of these cells. Methods HL-60 was treated with different concentrations of benzoquinone （BQ）. For testing cell viability, BQ-treated ceils were stained with trypan blue dye and counted. For proliferation evaluation, cells were stained with alamar blue dye and counted. HL-60 cells were also analyzed for their cell cycle distribution with a FACS sort flow eytometer. Results 3 μmol/L BQ did not significantly reduce cell viability as compared with the control cells, and the 3 μmol/L BQ-treated cell population showed the highest growth rate. The proportion of cells in S-phase at 3 μmol/L BQ- treated culture was higher than that of the control. Conclusion 3μmol/L BQ-treated HL-60 cells showed increased growth over the untreated control.

关 键 词：苯醌 HL-60 细胞增殖 白血病 

分 类 号：R135.1[医药卫生—劳动卫生] R994.3[医药卫生—公共卫生与预防医学]