新型姜黄素脂质体的制备和初步稳定性考察  

Preparation of Novel Curcumin Liposomes and associated Preliminary Stability Study

作　　者：孙晶[1,2] 季宇彬[1,2] 韩美华[3] 王向涛[3] 

机构地区：[1]哈尔滨商业大学/生命科学与环境科学研究中心药物研究所,哈尔滨150076 [2]国家教育部抗肿瘤天然药物工程研究中心,哈尔滨150076 [3]中国医学科学院药用植物研究所,北京100193

出　　处：《世界科学技术-中医药现代化》2008年第4期66-72,共7页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金(30772659):基于PAMAM的新型纳米粒的制备，负责人：王向涛;2007年药植所所长基金：基于树枝状聚合物的新型纳米给药系统，负责人：王向涛。

摘　　要：目的:制备包封率高、粒径小、稳定性好的新型姜黄素脂质体,并对其稳定性进行考察。方法:采用薄膜超声法制备新型姜黄素脂质体,以透射电镜(TEM)观察其形态,动态光散射(DIS)考察粒径分布,用超速离心法测定包封率,并考察其对温度、乙醇、酸碱的耐受性。结果:新型姜黄素脂质体粒径小,平均粒径97.08nm(多分散系数PDI为0.173),包封率为(99.37±0.89)%;稳定性试验证明新型姜黄素脂质体能够耐受热压灭菌(115℃,67kPa,30min)、最终浓度为40%的乙醇和最终浓度为1M的盐酸。结论:和传统脂质体相比,新型脂质体提高了姜黄素的包封率和稳定性,可以使药物更好地发挥作用。This paper introduces an approach to prepare novel curcumin liposomes featured with high encapsulation efficiency, small size, and good stability, and examines the associated stability. Novel curcumin liposomes were prepared by thin film-dispersion. The shape of liposomes were observed under a transmission electron microscopy （TEM）, and the partieles size evaluated through dynamic scattering （DLS）. The encapsulation efficiency was determined using centrifugal ultra-filtration. The novel curcumin liposomes were successfully prepared with an averaged diameter at 97.08nm. The encapsulation effieiency was 99.37±0.89%. The novel materials were tolerant to the steam sterilization （115℃, 67kPa,30min）, with the final concentration of ethanol at 40%, and Hydrochloride IM. It is believed that the novel curcumin liposome has an enhanced encapsulation efficiency and stability, compared with traditional eurcumin liposomes.

关 键 词：新型姜黄素脂质体 包封率 超速离心法 稳定性 

分 类 号：R944.9[医药卫生—药剂学] R256.34[医药卫生—药学]