EB病毒VCA-IgA抗体及病毒DNA载量在系统性红斑狼疮患者中的表达  

The expression of Epstein-Barr virus VCA-IgA antibody and virus DNA load in SLE patients

作　　者：叶志中[1] 尹志华[1] 陈杰[1] 罗秀霞[1] 

机构地区：[1]广东省深圳市福田区人民医院香蜜湖风湿病分院广东医学院深圳风湿病研究所,518040

出　　处：《实用医学杂志》2008年第20期3489-3491,共3页The Journal of Practical Medicine

基　　金：深圳医学重点学科建设资助项目(编号:2005C10);深圳市科技局资助项目(编号:200702012)

摘　　要：目的:检测EB病毒VCA-IgA抗体及病毒DNA载量在系统性红斑狼疮(SLE)患者外周血中的表达,探讨EB病毒与SLE的相关性。方法:分别采用酶联免疫吸附试验(ELISA)法和实时定量PCR方法检测EB病毒VCA-IgA抗体和EB病毒DNA载量。同时分析EB病毒VCA-IgA抗体与SLE患者的实验室指标抗Sm抗体、抗dsDNA抗体、补体C3和C4的相关性。结果:248例SLE患者中,49例EBV-VCA-IgA抗体阳性,167例健康对照者中12例阳性,SLE患者阳性率明显高于健康对照者(19.8%vs7.2%;P<0.001);SLE患者EB病毒载量也明显高于健康对照者。EB病毒VCA-IgA抗体阳性与抗Sm抗体、抗dsDNA抗体、补体C3和C4不相关。结论:EB病毒感染与SLE相关,EB病毒VCA-IgA抗体阳性者有较高的DNA载量,SLE的发病危险性亦高,EB病毒重新活化与SLE活动有关。Objective To examine the expressions of Epstein-Barr virus （EBV） VCA-IgA antibody and virus load in systemic lupus erythematosus （SLE） patients＇ peripheral blood, to explore the association between EBV infection and SLE occurrence and development. Methods EBV VCA-IgA antibody was determined by enzyme-linked immunosorbent assay （ELISA）. EB virus DNA load was quantified by real-time PCR （RT-PCR） The associations between the existence of EBV VCA-IgA antibody and laboratorial criterions such as anti-Sin antibody, anti-dsDNA antibody and C3, C4 in SLE patients were analyzed. Results EBV VCA-IgA antibody in 49/248 cases SLE patients were positive ,while 12/167 eases healthy controls were positive. The positive rate of EBV-VCA IgA antibody was higher in SLE patients than in controls （ 19.8% vs 7.2% ;P 〈 0.001 ）. EBV load was also significantly higher in SLE patients than in controls. There was no association between EBV-VCA-IgA antibody and anti-Sin antibody,anti-dsDNA antibody and C3,C4 （P 〉 0.05）. Conclusions EBV infection may be associated with SLE. People with EBV-VCA-IgA antibody positive expression have higher EBV DNA load and higher risk of SLE occurrence. EBV reactivation may contribute to the activation of SLE.

关 键 词：红斑狼疮 系统性 EB病毒 衣壳抗原IgA抗体 DNA载量 

分 类 号：R593.241[医药卫生—内科学]