晚熟甜橙芽变的ISSR鉴定  

The identification of Citrus sinensis cv.bud mutation by ISSR markers

作　　者：程昌凤 何桥[2] 洪林 郭启高[2] 漆巨容 吴纯清 余德全 魏召新 梁国鲁[2] 

机构地区：[1]重庆市农科院果树研究所,重庆江津402260 [2]西南大学园艺园林学院,重庆400716 [3]重庆长寿区农业局果经站,重庆长寿401220

出　　处：《西南农业学报》2008年第5期1378-1380,共3页Southwest China Journal of Agricultural Sciences

基　　金：重庆市自然科学基金资助项目(CSTC2004BB1027)

摘　　要：应用ISSR技术对晚熟甜橙伏令夏橙的大果无核枝、"安顺一号"等5个材料进行了鉴定。从100个引物中筛选出18个引物,共扩增出113条带,其中多态性条带数为15条,多态位点百分率为13.3%。结果表明:812、834、841引物对参试材料有明显扩增带差异,伏令夏橙大果无核枝与对照枝及伏令夏橙、"安顺一号"与伏令夏橙及锦橙在DNA分子水平上存在明显的遗传差异,晚熟甜橙大果无核枝为伏令夏橙一稳定芽变。ISSR技术能有效地鉴定晚熟甜橙芽变。DNA polymorphism of C. sinensis ev. were determined by inter simple sequence repeat （ISSR） molecular markers. 113 bands were amplified with 18 of 100 primers , among which 15 were polymorphic between mutants and parental cultivars, and the polymorphism ratio was 13.3%. These results showed that there were genetic diversity with primer 812,834 and 841 between seedless big fruit branch and the contrast branch , among ＇ Anshun No. 1 ＇ and Valencia and ＇ Jincheng＇ orange. Combining the results of morphological and molecular analysis, it was certified that the branch of C. sinensis cv. and ＇ Anshun No. 1 ＇ occurred genetic variation.

关 键 词：晚熟甜橙 变异 ISSR鉴定 

分 类 号：S666.4[农业科学—果树学]