饲料中环丙氨嗪和三聚氰胺含量的反相高效液相色谱测定方法  

Determination of cyromazine and melamine in feed by RP-HPLC

作　　者：魏瑞成[1] 郑勤[1] 陈明[1] 邵明诚[1] 王冉[1] 

机构地区：[1]江苏省农科院,江苏省畜禽产品安全性研究重点实验室,江苏南京210014

出　　处：《西南农业学报》2008年第5期1443-1446,共4页Southwest China Journal of Agricultural Sciences

基　　金：江苏省科技厅攻关项目BM2003306

摘　　要：本试验研究建立了饲料中环丙氨嗪和三聚氰胺残留量的反相高效液相色谱测定方法。样品均质后经20％氨水乙腈溶液提取、离心和氮吹富集浓缩等处理，在214nm波长下用二极管矩阵检测器检测。色谱柱为NH2柱，以乙腈：水溶液（97：3，V／V）为流动相，流速1．0mL／min。环丙氨嗪和三聚氰胺在0．025～5．0μg/mL浓度范围内与峰面积呈线性相关。在1．0、3．0、5．0和8．0mg／kg添加浓度范围内，平均回收率为74．04％～86．94％，相对标准偏差（RSD）为0．51％～2．21％，最低检测限为0．01μg／mL。本方法简便、快速，适用于饲料中环丙氨嗪和三聚氰胺多残留的检测。A method was developed for detecting cyromazine and melamine residues in feed by reversed-phase high performance liquid chromatography （RP-HPLC） with DAD detection at 214 nm. Cyromazine and melamine were extracted from feed with acetonitrile containing 20 % NH4 OH, centrifuged, then concentrated. A NH2 column was used. The mobile phase was acetonitrile and water （97： 3, V/V）, the flow rate was 1.0 mL/min. The peak area response was linear for cyromazine and melamine between 0. 025 - 5.0 μg/mL. Recoveries of cyromazine and melamine at fortified levels of 1.0,3.0,5.0 and 8.0 mg,/kg ranged from 74.04 % -86.94 % ,with relative standard deviation of 0.51 % -2.21%. The detection limit of both compounds was 0.01 μg/mL. The method was simple,rapid and suitable for determination of eyromazine and melamine residues in feed.

关 键 词：环丙氨嗪 三聚氰胺 反相高效液相色谱法 测定方法 

分 类 号：S816.17[农业科学—饲料科学]