CD4^+CD25^+调节性T细胞在特发性血小板减少性紫癜患者中的变化及意义  

Change and significance of CD4^+CD25^+ regulatory T cells in idiopathic thrombocytopenic purpura

作　　者：孙雨梅[1] 张彦明[1] 何广胜[2] 陆沭华[1] 韩雪花[1] 崔红霞[1] 

机构地区：[1]徐州医学院附属淮安医院江苏淮安市第二人民医院血液科,江苏淮安223002 [2]苏州大学附属第一医院血液科

出　　处：《临床血液学杂志》2008年第5期469-470,473,共3页Journal of Clinical Hematology

摘　　要：目的:通过检测特发性血小板减少性紫癜(ITP)患者治疗前后外周血CD4+CD25+调节性T细胞(regulatory T cell,Treg)的比例及变化规律,探讨Treg细胞在ITP发病中的可能作用。方法:采集30例急、慢性ITP患者治疗前、后和20例正常对照者的外周血标本,流式细胞仪检测Treg细胞的比例及变化,并评价其与血小板计数的相关性。结果:30例ITP患者治疗前Treg细胞的比例为(1.59±0.86)%,明显低于正常对照组(3.87±1.73)%(P<0.01),治疗后其比例显著升高,为(2.51±1.17)%,明显高于治疗前(P<0.01),但仍低于正常对照组(P<0.01);Treg细胞在治疗显效和良效组显著高于进步和无效组(P<0.01),进步组与无效组比较差异无统计学意义(P>0.05);此外治疗前后Treg细胞比例与血小板计数呈显著正相关(P<0.05)。结论:ITP患者外周血Treg细胞比例降低,但随免疫抑制治疗的疗效逐渐升高,提示CD4+CD25+调节性T细胞可能参与了ITP的发病机制。Objective： To explore the role of CD4^＋ CD25^＋ regulatory T cells in pathophysiology of idiopathic thrombocytopenic purpura（ITP） by analyzed the rate in ITP patients. Method： Peripheral blood mononuclear cells （PBMNCs） from 30 cases of ITP patients and 20 cases of health controls were collected and the population of CD4^＋ CD25^＋ regulatory T cells was detected by flow cytometry. The correlation between CD4^＋ CD25^＋ regulatory T cells and platelet counts was analyzed. Result： The proportion of CD4^＋ CD25^＋ regulatory T cells in ITP patients before therapy was decreased remarkably than that of health control group （ P 〈0.01）, the rate significantly higher after therapy than that of patients before therapy （ P〈0.01）, but less than that of health control group （ P 〈0.01）. The proportion of CD4^＋ CD25^＋ regulatory T cells in the effective therapy group was higher than that of in the refractory group （ P 〈0.01）. No significant difference between the progressing therapy group and no effective therapy group （ P 〉0.05）. There was a significant correlation between CD4^＋ CD25^＋ regulatory T cells and platelet counts （ P 〈0.05）. Conclusion：The low percentage of CD4^＋ CD25^＋ regulatory T cells in ITP patients may be involved in pathophysiology of ITP.

关 键 词：紫癜 血小板减少性 调节性T细胞 CD4^+CD25^+ 

分 类 号：R554[医药卫生—血液循环系统疾病]