HPLC测定参术儿康糖浆中2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量  

Determination of 2,3,5,4'-Tetrahydroxystilbene-2-O-β-D-glucoside in Shenzhu Erkang Syrup by HPLC

作　　者：商量[1] 章春宇[2] 李红岩[3] 商慧娟[4] 王威[3] 

机构地区：[1]吉林省肿瘤医院,长春130012 [2]长春医学高等专科学校,长春130051 [3]吉林省中医中药研究院,长春130021 [4]吉林省药品检验所,长春130062

出　　处：《中国现代应用药学》2008年第5期436-438,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立高效液相色谱法测定参术儿康糖浆中2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量。方法采用TechsphereC18色谱柱;流动相为乙腈-水(16∶84);检测波长为320nm;柱温为40℃。结果2,3,5,4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷进样量在0.04496～0.22480μg内与峰面积积分值呈良好的线性关系(r=0.9999);平均加样回收率为100.25%(RSD=0.69%,n=5)。结论方法简便、准确,可作为该制剂的质量控制方法。OBJECTIVE To establish an HPLC method for the determination of 2,3,5,4＇-tetrahydroxystilbene-2-O-β-D- glucoside in Shenzhu Erkang syrup. METHODS The Techsphere C18 column was used as the stationary phase with the mobile phase of acetonitrile-water（ 16： 84）. The detection wavelength was 320 nm and the column temperature was 40 ℃. RESULTS The linear range of 2,3,5,4 ＇ -tetrahydroxystilbene-2-O-β-D-glucoside was 0. 044 96 -0. 224 80 μg（ r = 0. 999 9）. The average recovery was 100.25% with the RSD of 0.69% （n = 5）. CONCLUSION The method is convenient and accurate. It ean be used for the quality control of 2,3,5,4＇ -tetrahydroxystilbene-2-O-β-D-glueoside in Shenzhu Erkang syrup.

关 键 词：2 3 5 4’-四羟基二苯乙烯-2-O-β-D-葡萄糖苷 参术儿康糖浆 高效液相色谱法 

分 类 号：R917.792[医药卫生—药物分析学] R917.101[医药卫生—药学]