香蕉穿孔线虫28S rRNA基因的D2／D3区序列分析  

Phylogentic Analysis of Radopholus similis from D2 and D3 Fragments of the 28S rRNA Gene Sequences

作　　者：李佳[1,2] 彭德良[1] 黄文坤[1] 

机构地区：[1]中国农业科学院植物保护研究所／植物病虫害生物学国家重点实验室,北京100193 [2]中国农业科学院研究生院,北京100081

出　　处：《华中农业大学学报》2008年第5期585-589,共5页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金(30671363);国家“十一五”科技支撑计划(2006BAD08A14,2006BAD08A08);公益性行业(农业)科研专项(ny-hyzx07-050)资助

摘　　要：采用线虫通用引物D2A和D3B对9个香蕉穿孔线虫种群的核糖体DNA28S大亚基的D2/D3区进行了扩增,获得的片段长度约为780bp,克隆测序后使用UPGMA法进行聚类分析和构建系统发育树。结果表明9个线虫种群D2/D3区核苷酸序列相似性为99.13%,其中8个群体的序列与越南报道的香蕉穿孔线虫近源种(Radopholus sp.7B VietNam)的D2/D3区核苷酸序列(DQ328712)相似性为97.46%,说明香蕉穿孔线虫D2/D3区具有较大的保守性。聚类分析结果表明,RSHN12r、RSSH、RSHL、RSHK、RSLZ、RSSZ、RSHN13、RSHN12p等8个种群聚为一类,亲缘关系很近,RSHN3群体与以上8个群体亲缘关系较远,说明RSHN12r、RSSH、RSHL、RSHK、RSLZ、RSSZ、RSHN13、RSHN12p这8个香蕉穿孔线虫种群可能来源于同一地理种群,而RSHN3种群可能来源于另一个地理种群。The amplification of D2 and D3 fragments of the 28S rRNA gene from Radopholus sirnilis populations detected from imported seedling and horticultural plants using universal primers（D2A and D3B） yielded one fragment about 780 bp. The results of D2 and D3 fragment sequence analysis and the alignment between the nine populations and related species Radopholus and Pratylenchus using UPGMA method showed that eight populations（RSHN12rR, RSSH, RSHL, RSHK, RSLZ, RSSZ, RSHN13） were clustered in the same group and the similarity between them was 99. 13%, while they were compared with the sequence of Radopholus sp. 7B VietNam（DQ328712）, the similarity was 97.46%. The results of phylogentic analysis with UPGMA program showed that there were two main branches, the first branch contained eight populations of R. similis （RSHN12rR, RSSH, RSHL, RSHK, RSLZ, RSSZ, RSHN13,RSHN12p）, they may have the same origin. And the second branch comprised of only one population（RSHN3） which may have another origin.

关 键 词：香蕉穿孔线虫 28S RRNA基因 D2/D3扩展区域 

分 类 号：S432.45[农业科学—植物病理学]