反硝化细菌的分离筛选及其反硝化特性的初步研究  

Screening of Denitrobacteria and Preliminary Study about Its Denitrification Properties

作　　者：曾庆武[1] 梁运祥[1] 葛向阳[1] 

机构地区：[1]华中农业大学农业微生物学国家重点实验室,武汉430070

出　　处：《华中农业大学学报》2008年第5期616-620,共5页Journal of Huazhong Agricultural University

基　　金：国家"863"高技术研究发展计划项目(2003AA601060)资助

摘　　要：从不同的水样、土样中用反硝化选择性培养基分离出202株反硝化细菌。以硝酸盐的降解、亚硝酸盐的积累和脱氮率为筛选指标,从这些菌株中得到1株反硝化能力强的菌株A13,经生理生化试验和16SrDNA序列分析,鉴定该菌株为地衣芽胞杆菌(Bacillus licheniformis)。然后将该菌株与保存的反硝化菌DNF409联合应用,脱氮率比应用单株菌提高近30%。在此基础上采用响应面分析法(中心组合一致精度设计,SAS9.1.3),建立了初始硝态氮浓度为25mg/L水样脱氮率的回归方程,同时得出最佳反硝化条件是CODMn为35.1mg/L,温度为32.5℃,投菌量为6.2×106cfu/mL,反硝化时间为114.2h,此时脱氮率达99%以上。Two hundred and two strains of denitrobacteria were isolated from different soil and hquid samples through denitrification medium. A strain named A13 was obtained for its great capability of denitrification by measuring degradation of nitrate,accumulation of nitrite and percentage of denitrification. A13 was identified as Bacillus licheniformis by physiological and biochemical experiment and analysis of its 16S rDNA sequence. Compared with single strain, the combination application of DNF409 （collected in this lab） and A13 increased the percentage of denitrification about 30%. An equation of percentage of denitrification was established by response surface analysis （uniform precision of central composite design, SAS 9. 1.3） in the case of combination application about DNF409 and A13. The result showed that the optimum denitrification condition is as follows：CODMn 35.1 mg/L, temperature 32.5 ℃, bacterial concentration 6. 2×10^6 cfu/mL,time 114. 2 h.

关 键 词：反硝化 硝酸盐 亚硝酸盐 响应面分析 筛选 

分 类 号：Q939.9[生物学—微生物学]