补骨脂素加长波紫外线诱导HL-60细胞凋亡时Caspase8、Caspase3蛋白的变化  

Changes of Caspase8 and Caspase3 Protein in HL-60 Cells during Apoptosis Induced by PUVA

作　　者：陈楠楠[1] 尤婷玉[1] 向阳[1] 张德杰[1] 黄世林[1] 

机构地区：[1]中国人民解放军第210医院中医血液科,辽宁大连116021

出　　处：《中国中医药信息杂志》2008年第11期27-29,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(30472257)

摘　　要：目的研究补骨脂素(PSO)加长波紫外线(UVA)光化学疗法(PUVA)诱导人白血病HL-60细胞凋亡时Caspase8、Caspase3蛋白的变化。方法用不同浓度的中药补骨脂中提取的PSO和/或不同照射时间、波长为360nm的UVA作用于HL-60细胞,检测细胞凋亡率,在电镜下观察细胞超微结构改变及Caspase8、Caspase3蛋白的表达,用多因素方差分析法对定量资料进行统计分析。结果PSO、UVA照射及PUVA均可使细胞凋亡率增加,上调Caspase8、Caspase3蛋白的表达,PUVA的作用显著强于前两者;PUVA处理后的HL-60细胞超微结构出现明显的凋亡形态学改变。结论PUVA在诱导HL-60细胞凋亡的过程中激活了Caspase8、Caspase3。Objective To study the changes of Caspase8 and Caspase3 protein in HL-60 cells during apoptosis induced by psoralen （PSO） plus ultraviolet A （UVA）. Methods HL-60 cells were treated with PSO, extracted from Chinese medicine psoralea fruits in different concentrations, plus UVA of wave length 360 nm in different irradiation time. The apoptosis ratios, ultrastructure changes and the expression of Caspase8 and Caspase3 protein were detected. The factorial design and analysis of variance were used to analyze the interaction among the factors. Results PSO, UVA and PUVA all increased the apoptosis ratios and up-regulated the expression of Caspase8 and Caspase3 protein, but the effects of PUVA were the strongest. There were obvious changes about apoptosis under the electron microscope after treatment with PUVA. Conclusions PUVA can induce the apoptosis of HL-60 cells and activate Caspase8 and Caspase3 gene.

关 键 词：补骨脂素 PUVA HL-60细胞 凋亡 CASPASE8 CASPASE3 

分 类 号：R285.5[医药卫生—中药学]