中药复方921粗提物抗肿瘤作用机理研究  

Mechanism of Anticancer Activities of the Extracts of Prescription 921

作　　者：厉将斌[1] 李洪燕[2] 郭晓健[3] 陈晓光[2] 叶祖光[4] 郭应禄[3] 

机构地区：[1]北京大学光华管理学院卫生经济与管理系,北京100871 [2]中国医学科学院中国协和医科大学药物研究所,北京100050 [3]北京大学第一医院泌尿外科,北京100034 [4]中国中医科学院,北京100700

出　　处：《中国中医药信息杂志》2008年第11期35-37,共3页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的探索中药复方921粗提物抗肿瘤药效的作用机理。方法用大分子生物合成法、细胞周期法、Western Blot法,检测中药复方921粗提物在体外对人前列腺癌细胞PC-3M生长抑制作用。结果中药复方921粗提物对肿瘤细胞的DNA合成有抑制作用,经72h作用后能将细胞阻滞于S期,并能诱导S期相关蛋白CyclinA表达下调。结论921方抗肿瘤作用的机理可能与抑制肿瘤细胞DNA合成、诱导CyclinA蛋白表达下调并进而导致细胞周期S期阻滞有关。Objective To investigate the mechanism of anticancer activities of the extracts of prescription 921. Methods The inhibitory effects of the extracts of prescription 921 on DNA and RNA biosyntheses in cultured prostate PC-3M were investigated with ^3H-TdR or ^3H-UR incorporation. Flowcytometry was applied to analyze the regulating cell cycle of the extracts of prescription 921. The expression of cell cycle related factors CyclinA, CyclinB, p21 and p27 were detected by Western-blot. Results The extracts of prescription 921 showed inhibitory effect on DNA biosyntheses. The ceUs of PC-3M were arrested in S-phage when treated with the extracts for 72 h. The expression level of CyclinA was down-regulated while expression of CyclinB, p21 and p27 was not altered. Conclusion The extracts of prescription of 921 play its anticancer activities by inhibiting DNA biosyntheses and arresting the cells in S-phage by down-regulating the expression of Cyclin A.

关 键 词：中药复方921 抗肿瘤 机理研究 

分 类 号：R285.5[医药卫生—中药学]