重庆稻瘟病菌群体遗传多样性分析  

Population Structure of Magnaporthe grisea from Rice in Chongqing

作　　者：杨水英[1] 韩海波[2] 孙现超[1] 邸仕忠[2] 熊艳[1] 青玲[1] 

机构地区：[1]西南大学植物保护学院,重庆400715 [2]重庆市涪陵区农业科学研究所,重庆408000

出　　处：《河南农业科学》2008年第11期76-79,共4页Journal of Henan Agricultural Sciences

基　　金：重庆市自然科学基金项目(CSTC,2006BB1318)

摘　　要：采用rep-PCR指纹技术，对73个重庆稻瘟病菌菌株进行了DNA指纹扩增。结果表明，菌株间显示了DNA指纹的多态性，供试菌株分别扩增出2～17条DNA带。经UPGMA聚类分析，在0．80遗传相似水平下，供试菌株分为12个遗传谱系，其中谱系L7，L9，L12为优势谱系。重庆稻瘟病菌的群体结构呈现多样性和复杂性，菌株的遗传谱系与原寄主品种和地理分布之间均表现出较明显的相关性。DNA fingerprints of 73 Magnaporthe grisea isolates collected from the rice in Chongqing were tested with the rep-PCR technique. Polymorphism of DNA fingerprints existed among the isolates. The tested isolates showed 2-17 DNA bands after amplification. UPGMA analysis clustered 73 isolates into 12 genetic lineages at 0.8 genetic similarity. Of these lineages, LT, L9 and L12 were dominate lineages. The results indicated that genetic structure of M. grisea population in Chongqing was multiplicity and complexity, and the results also revealed that genetic lineage isolates were directly related to variety and geography distribution.

关 键 词：稻瘟病菌 遗传多样性 rep—PCR 

分 类 号：S435.114.41[农业科学—农业昆虫与害虫防治]