猪神经介素B和受体基因的克隆与组织分布  

cDNA Cloning and Tissue Distribution of Porcine Neuromedin B and Its Receptor

作　　者：王静[1] 李宏基[1] 王伟杰[1] 武宇晓[1] 李平[1] 张志强[1] 台玉磊[1] 臧猛[1] 杨国宇[1] 

机构地区：[1]河南农业大学动物生理生化实验室,河南郑州450002

出　　处：《河南农业科学》2008年第11期126-130,共5页Journal of Henan Agricultural Sciences

基　　金：河南省重点科技攻关项目(0523010500)

摘　　要：基于电子延伸序列,克隆并分析了猪神经介素B(NMB)和受体(NMBR)基因。猪NMBcDNA克隆片段长度为567 bp,开放阅读框架长度为366 bp,编码121个氨基酸。NMBR cDNA克隆片段长度为1 194 bp,开放阅读框架长度为1 173 bp,编码390个氨基酸。同源分析表明,猪NMB的核酸序列与牛、人、小鼠和大鼠的同源性分别为87.1%,85.2%,78.1%和76.6%,氨基酸序列的同源性分别为81%,74.4%,70.2%和70.1%;NMBR的核酸序列与牛、人、小鼠和大鼠的同源性分别为91%,89%,84.2%和83.6%,氨基酸序列的同源性分别为92.3%,90.3%,86.9%和86.4%。组织分布结果显示,猪NMB在多种组织均有分布,NMBR仅分布在大脑。克隆的猪NMB和受体基因分别注册GenBank(EU375564和EU670045)。The coding sequence of porcine Neuromedin B and its receptor gene were amplified based on the in silico sequence information in the present study. DNA sequencing and bioinformatic analysis showed that the porcine Neuromedin B gene was 567 bp long,has one open reading frame of 366 bp and encodes 121 amino acids. The length of Neuromedin BR gene was 1194 bp,which containing one open reading frame of 1173 bp and encoding 390 amino acids. Compared to bovine,human, mouse and rat,the nucleotide homology of porcine Neuromedin B was 87.1% ,85.2% ,78.1% and 76.6% ,respectively. The homology of amino acid sequence was 81% ,74.4% ,70.2% and 70.1%, respectively. As for the gene of porcine Neuromedin BR,the nucleotide homology was 91% ,89%, 84.2% and 83. 6%, while the amino acid sequence homology was 92. 3%, 90. 3%, 86.9% and 86.4%, respectively. Semi-quantitative RT-PCR analysis showed that the neuromedin B gene ubiquitously expressed in various tissues tested,while the gene of Neuromedin BR preferentially expressed in the brain. The sequences of porcine Neuromedin B and its receptor gene were submitted to the GenBank （EU375564 and EU670045）.

关 键 词：猪 NMB 受体 克隆 序列分析 组织分布 

分 类 号：S828[农业科学—畜牧学]