半乳糖凝集素-2基因变异与急性冠状动脉综合征风险关联研究  

Relationship Between Genetic Variation of Galectin-2 Gene and Risk of Acute Coronary Syndrome

作　　者：赵文强[1] 王俊[1] 谢红珍[1] 欧阳星文[1] 程辉[1] 吴艳君[1] 

机构地区：[1]江苏大学第四附属医院心内科,江苏省镇江市212003

出　　处：《中国循环杂志》2008年第5期360-363,共4页Chinese Circulation Journal

基　　金：镇江市科技局社会发展基金资助项目(编号:SH2006048)

摘　　要：目的:研究半乳糖凝集素-2(LGALS2)基因变异与急性冠状动脉综合征(ACS)惟患风险之间的关系。方法:采用聚合酶链反应—限制性片段长度多态性法对248例ACS组患者、212例稳定性心绞痛组患者和138例对照组患者检测LGALS2基因C3279T位点基因型、基因型频率,等位基因、等位基因频率,用冠状动脉造影测定病变血管支数和狭窄程度积分,用酶联免疫吸附法测试血浆淋巴毒素α和血管细胞黏附分子-1水平;用散射比浊法测试血浆C反应蛋白水平;探索基因型变异与ACS风险、病变血管支数、狭窄程度积分和3个细胞因子水平之间的关系。结果:ACS组与对照组比TT基因型频率和T等位基因频率显著减低(P<0.01和P<0.001),差异有统计学意义;稳定性心绞痛组与对照组比TT基因型频率和T等位基因频率差异无统计学意义(P>0.05)。CT+TT基因型发生ACS的危险性与CC基因型发生ACS的危险性比(OR=0.73,P<0.01)、T等位基因发生ACS的危险性与C等位基因发生ACS的危险性比(OR=0.67,P<0.001)差异均有统计学意义;但CT+TT基因型发生稳定性心绞痛的危险性与CC基因型发生稳定性心绞痛的危险性比(OR=0.84,P>0.05)、T等位基因发生稳定性心绞痛的危险性与C等位基因发生稳定性心绞痛的危险性比(OR=0.84,P>0.05)差异均无统计学意义。基因型与冠状动脉病变程度:TT基因型与CC基因型比血管病变支数频率和狭窄程度积分均显著降低(P<0.05和P<0.001)。基因型变异与炎性细胞因子:TT基因型与CC基因型比血浆淋巴毒素α、C反应蛋白、血管黏附分子-1水平均显著降低(均P<0.001)。结论:LGALS2基因C3279T位点变异对ACS易患风险、血管病变程度和炎症级联反应起保护作用。Objective：To study the relationship between variation of galeetin-2 gene （LGALS2）and the risk of acute coronary syndrome （ACS）. Methods：Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） was used to detect C3279T SNP in LGALS2 in three groups：ACS group：n =248, stable angina pectoris（SAP） group：n =212, and control group：n = 138. Diseased vessel numbers （DVN）and stenostie severity integral（SSI） were examined by coronary angiogram;serum levels of inflammatory cytokines including lymphotoxin-α （ LTA）, vascular cell adhesion molecule-1 （ VCAM-1 ） and C-reactive protein （CRP） were measured by ELLISA method ; and nephelometry was also tested. The relationship between genotype variants and ACS risk, DVN, SSI and plasma concentrations of 3 inflammatory cytokines were investigated. Results ： Compared with the control group, CT ＋ Tr genotype and T allele showed significant low frequencies in ACS group（ P 〈0. 01 and P 〈0. 001 ）. Odds ratios for ACS risk in CT ＋ TT genotype and T allele were respectively lower than in CC genotype and C allele（ OR = 0. 73 ,P 〈 0. 01;OR = 0. 67, P 〈 0. 001 ） , but variant genotype and T allele were not obviously related to SAP risk（ P 〉 0. 05 ）. DVN, SSI and plasma concentrations of 3 eytokines in TF genotype were significantly lower than those in CC genotype （ all P 〈 0. 05 ）. Conclusion：3279C/T variation in LGALS2 had the protective role against ACS susceptibility risk, extent of vascular lesion and the inflammation cascade reaction.

关 键 词：冠状动脉疾病 基因多态性 基因型 易感性 

分 类 号：R54[医药卫生—心血管疾病]