苍术苷对七氟醚后处理诱导脑保护作用的影响  

The effect of Atractyloside on sevoflurane postconditioning in focal cerebral ischemia

作　　者：夏明[1] 李伟彦[1] 董海龙[2] 熊利泽[2] 

机构地区：[1]南京军区南京总医院麻醉科,江苏南京210002 [2]第四军医大学西京医院麻醉科,陕西西安710032

出　　处：《医学研究生学报》2008年第10期1030-1033,共4页Journal of Medical Postgraduates

基　　金：国家自然科学基金资助项目(批准号:30471664)

摘　　要：目的:观察苍术苷对七氟醚后处理诱导脑保护作用的影响。方法:清洁级雄性SD大鼠50只,随机分为5组:对照组、七氟醚后处理组、七氟醚+苍术苷组、七氟醚+溶剂组和苍术苷组。每组10只大鼠。采用大脑中动脉线栓法阻闭(MCAO)120 min后再灌注72 h,制备局灶性脑缺血模型。再灌注即刻的前20 min和后10 min吸入七氟醚。七氟醚后处理前给予苍术苷侧脑室注射。再灌注后的24、48和72 h行神经行为学评分,并于最后一次评分后测定脑梗死容积比。结果:缺血-再灌注72 h后,与对照组脑梗死容积比(0.55±0.07)相比,七氟醚后处理组(0.32±0.05)明显降低(P=0.000);七氟醚+苍术苷组(0.57±0.06,P=0.418)和苍术苷组(0.56±0.05,P=0.605)均无统计学差异,七氟醚+苍术苷组和苍术苷组分别与七氟醚后处理组相比,差异有显著性统计学意义(P=0.000);七氟醚+溶剂组(0.35±0.06,P=0.218)与七氟醚后处理组相比差异无显著性意义。各时间点神经功能评分,七氟醚后处理组和七氟醚+溶剂组明显优于对照组(P<0.05)。七氟醚+苍术苷组和苍术苷组明显低于七氟醚后处理组(P<0.05)。结论:苍术苷消除了七氟醚后处理的脑保护作用。Objective： To investigate the effect of Atractyloside on sevoflurane postconditioning in focal cerebral ischemia.Methods： Fifty male SD rats were randomly assigned to five groups（n=10 each）： control group（con）,1.0 MAC group（sevo）,1.0 MAC＋ Atractyloside group（sevo＋Atr）,1.0 MAC＋ vehicle group（sevo＋vehicle）and Atractyloside group（Atr）.All animals were subjected to the right middle cerebral artery occlusion（MCAO） for 120 min followed by reperfusion for 72 h.The animals in sevo groups were given 1.0 MAC sevoflurane inhalation from 20 min before to 10 min after reperfusion.The animals in Atr groups were given Atractyloside by ICV injection before sevoflurane postcondioning.The neurological deficit scores（NDS） were recorded at 24 h,48 h,and 72 h after reperfusion.Infarct volume percentage was determined after the last NDS assessment.Results： The infarct volume percentage ratio of sevo group（0.32±0.05） was significantly lower than that of con group（0.55±0.07）（P〈0.05）.The infarct volume percentage of Sevo ＋Atr group（0.57±0.06）and Atr group（0.56±0.05） were not significantly different from that of con group（P〉0.05）.The infarct volume percentage ratio of sevo＋Atr group and Atr group were significantly higher than that of Sevo group（P〈0.05）.Sevo＋vehicle group（0.35＋0.06） was not significantly different from that of Sevo group（P〉0.05）.NDS of 24 h,48 h and 72 h after reperfusion in sevo group and sevo＋vehicle were significantly higher than that in Con group.NDS of 24 h,48 h and 72 h after reperfusion in sevo＋Atr group and Atr were significantly lower than that in Sevo group.Conclusion： Atractyloside abolished the protective effects of sevoflurane postconditioning on focal cerebral ischemia-reperfusion injury.

关 键 词：七氟醚 缺血-再灌注损伤 后处理 大脑 苍术苷 线粒体转换孔 

分 类 号：R971.2[医药卫生—药品]