高浓度软脂酸体外抑制人外周血内皮祖细胞的增殖  

High-concentration palmitic acid inhibits the proliferation of peripheral blood-derived human endothelial progenitor cells in vitro

作　　者：江海龙[1] 梁春[1] 潘晓明[1] 谭鸿兵[1] 吴建祥[1] 孙承波[1] 吴宗贵[1] 

机构地区：[1]第二军医大学长征医院心内科,上海200003

出　　处：《南方医科大学学报》2008年第10期1786-1788,1801,共4页Journal of Southern Medical University

基　　金：国家重点基础研究规划项目973基金(2005CB523309)

摘　　要：目的观察软脂酸对体外培养的人外周血内皮祖细胞(EPCs)增殖的影响。方法采用密度梯度离心法分离培养人外周血单个核细胞,经FITC标记荆豆凝集素I(FITC-UEA-I)和Dil标记的乙酰化低密度脂蛋白(Dil-acLDL)双染色鉴定为正在分化的EPCs,并进一步通过流式细胞仪检测其表面标志CD34、CD133和VEGFR2。分别以不同浓度(0、50、100、200、400、800μmol/L)软脂酸溶液作用48h,以及400μmol/L软脂酸溶液作用不同时间(0、12、24、36、48和60h)后,CCK-8法观察EPCs增殖的情况。结果与对照组比较,400μmol/L组及800μmol/L软脂酸组均抑制了EPCs的增殖功能(P<0.05),在400μmol/L时最为显著;且400μmol/L软脂酸对EPCs增殖抑制作用随时间延长逐渐增强,48h达到高峰(增殖抑制率为58.59%(P<0.05)。结论高浓度软脂酸体外能显著抑制外周血内皮祖细胞的增殖。Objective To study the effects ofpalmitic acid （PA） on the proliferation of peripheral blood-derived endothelial progenitor cells （EPCs） in vitro. Methods The mononuclear cells （MNCs） were isolated fi＇om the peripheral blood by Ficoll density-gradient centrifugation. The isolated EPCs were characterized by Di-LDI uptake and FITC-lectin binding assay using laser confocal microscope, and further identified by detection of CD34, CD133 and VEGFR2 expression using flow cytometry. The cultured EPCs were incubated in the presence of PA at the concentrations of 0, 50, 100, 200, 400 and 800 μmol/L for different durations （0, 12, 24, 36, 48 and 60 h）. The cell morphology was observed and cell proliferation determined with CCK-8 assay. Results Incubation with 400 and 800 μmol/L of PA significantly inhibited the proliferative ability of EPCs as compared with the control group （P〈0.05）. PA at 400 μmol/L had the strongest effect on the cell proliferation, and this effect was intensified with the passage of time, reaching the peak at 48 h with the growth inhibition rate of 58.59% （P〈0.05）. Conclusion High-concentration PA can significantly inhibit the proliferation of EPCs in vitro.

关 键 词：软脂酸 内皮祖细胞 细胞增殖 

分 类 号：R543[医药卫生—心血管疾病]