涤痰化瘀药对高血压大鼠及左室心肌细胞周期及心室重构的影响  

Effects of phlegm-dispelling and blood stasis-resolving traditional Chinese drugs on the blood pressure and cell cycle of left ventricular cardiac myocytes in hypertensive rats

作　　者：陈茹琴[1] 熊平[2] 周欣欣[3] 梁兆球[1] 吴良芝[1] 

机构地区：[1]广东省第二人民医院,广东广州510317 [2]华南农业大学,广东广州510642 [3]广州中医药大学,广东广州510405

出　　处：《南方医科大学学报》2008年第10期1806-1809,共4页Journal of Southern Medical University

基　　金：广东省自然科学基金(5003905)

摘　　要：目的研究涤痰化瘀药对高血压模型大鼠心肌细胞周期及左室重构的影响。方法设正常对照组(n=8)、假手术组(n=8)、模型组(n=10)和中药高剂量组(n=11)、低剂量组(n=11),采用二肾二夹法建立高血压大鼠模型,用大鼠血压计测尾动脉收缩压,模型成功后用药60 d,测大鼠左室质量(LVM)、左室质量指数(LVI),流式细胞仪测定心肌细胞周期。结果模型组血压和LVI显著高于正常对照组和假手术组(P<0.05,P<0.01),经涤痰化瘀药治疗后,中药低剂量组和高剂量组的血压和LVM有明显下降趋势,LVI与模型组比较有显著差异(P<0.05)。模型组G0/G1期细胞显著减少,S期细胞显著增多;中药低剂量组和高剂量组G0/G1期细胞显著高于模型组(P<0.05),S期细胞显著低于模型组(P<0.05),与正常组和假手术组无显著差异(P>0.05),中药低剂量组与高剂量组之间无显著差异(P>0.05)。各组G2/M期细胞无显著差异(P>0.05)。结论涤痰化瘀药可逆转高血压左室重构,使高血压左室心肌细胞停滞于G0/G1周期相,阻止细胞进入S期。其逆转高血压大鼠左室肥厚(LVH)及改善高血压预后的作用与负调控心肌细胞周期、抑制细胞分裂和增殖有关。Objective To observe the effects ofphlegm-dispeUing and blood stasis-resolving traditional Chinese drugs on the cell cycle of cardiac myocytes and left ventricular reconstruction in hypertensive rats. Methods Bilateral renal artery stenosis was conducted to induce hypertension in rats, which were randomly divided into hypertensive model group （n=10）, sham-operated group （n=8）, high-dose drug group （n=11） and low-dose drug group （n=11）, with 8 normal untreated rats as the normal control group. The systolic blood pressure （SBP） was measured in the tail artery of the rats. Two months after the operation, the left ventricular mass （LVM） and LVM index （LVI） were calculated in all the rats. The cell cycle changes in the left ventricular cardiac myocytes were evaluated using flow cytometry. Results The mean blood pressure and LVI of the hypertensive model group were significantly higher than those of the normal control （P〈0.05） and sham-operated group （P〈0. 01）. After treatment with preparation of the traditional Chinese drugs at either high or low dose, the mean blood pressure and LVM of the rats showed obvious reduction, and LVI was decreased significantly compared with that of the model group （P〈0. 05）. Compared with the hypertensive model group which showed obviously decreased cell percentage in G0/G1 phase and increased S phase cells, the treatment at both doses significantly increased the cells in G0/G1 phase （P〈0.05） and decreased the S-phase cells （P〈0.05） to levels comparable to those in the normal control and sham-operated groups （P〉0.05）. The percentage of G2/M-phase cells showed no significant difference between the groups （P〉0.05）. Conclusion The traditional Chinese drugs can significantly decrease blood pressure and LVI in hypertensive rats, and induce Cell cycle arrest in G0/G1 phase to reverse left ventricular hypertrophy by regulating the cell cycle and inhibiting the division and proliferation of the cardiac myocytes.

关 键 词：涤痰化瘀 二肾二夹 高血压 左心室肥厚 细胞周期 

分 类 号：R285.5[医药卫生—中药学]