肠炎清对大鼠溃疡性结肠炎肠粘膜介素-10和细胞间黏附分子-1  

Effects of Changyanqing decoction on the expressions of interleukin-10 and intercellular adhesion molecule-1 in rats with ulcerative colitis

作　　者：吕永慧[1] 宋卫兵[2] 肖冰[2] 龙燕[3] 姬宏莉[2] 王媛媛[2] 张小兰[2] 李鹰飞[2] 

机构地区：[1]广州市中医医院,广东广州510000 [2]南方医科大学南方医院消化病研究所,广东广州510515 [3]湖南会同县中医院,湖南会同4108300

出　　处：《南方医科大学学报》2008年第10期1891-1893,1896,共4页Journal of Southern Medical University

摘　　要：目的研究肠炎清对大鼠溃疡性结肠炎肠粘膜细胞间的黏附分子-1(ICAM-1),白介素-10(IL-10)表达的影响,探讨肠炎清的抗炎机制。方法应用三硝基苯磺酸((TNB)/乙醇灌肠,制备大鼠溃疡性结肠炎模型,实验设正常对照组,模型对照组,柳氮磺吡啶(SASP)组(100mg/kg),肠炎清组(39.75mg/kg),每天灌肠及灌胃给药1次,给药时间从造模后第2天开始至实验结束共7d,第4天,第7天分别观察大鼠疾病活动指数(DAI)和结肠黏膜损伤指数(CMDI)。免疫组化法检测大鼠肠黏膜ICAM-1蛋白的表达。采用酶联免疫吸附法测IL-10,生化法检测大鼠结肠组织髓过氧化酶(MPO)活性。结果与正常组相比,模型组DAI、CMDI、HS评分及结肠组织MPO活性明显升高(P<0.01)。结肠黏膜ICAM-1表达明显增强(P<0.01),IL-10表达减弱(P<0.01)。肠炎清组DAI,CMDI,HS评分及MPO活性较同期模型组有明显下降(P<0.01),ICAM-1表达也有不同程度降低(P<0.01),IL-10表达增强(P<0.01)。大鼠结肠黏膜IL-10与ICAM-1表达呈正相关(r=0.911,P<0.01),ICAM-1与MPO活性也呈正相关(r=0.621,P<0.01)。结论肠炎清对大鼠溃疡性结肠炎有保护作用,其作用机制可能与增强IL-10、减少黏附分子ICAM-1产生以及降低中性粒细胞浸润有关。Objective To investigate the effect of Changyanqing decoction, a traditional Chinese medicinal preparation, on the expressions of interleukin-10 （IL-10） and intercellular adhesion molecule-1 （ICAM-1） in the colon mucosa of rats with ulcerative colitis. Methods The rats with ulcerative colitis induced by trinitrobenzene sulphonic acid and ethanol enema were randomly divided into 3 groups, namely the model group, sulfasalazine （SASP） group, and Changyanqing decoction group. Daily treatment with intragastric administration and enema of normal saline, SASP （100 mg/kg）, and Changyanqing decoction （39.75 mg/kg）, respectively, were administered 24 h after the establishment of colitis till the end of the experiment. Another group of rats was used as the normal control group. The disease activity index （DAI） and colon mucosa damage index （CMDI） of the rats were calculated. The activity of myeloperoxidase （MPO） was measured by biochemical method, and the expressions of IL-10 and ICAM-1 protein were measured by ELISA and irnmunohistochemistry, respectively. Results Compared with the normal group, the model group showed significantly increased DAI, CMDI, HS score and MPO activity in the colon tissues （P〈0.01）, with also significantly increased expression of ICAM-1 （P〈0.01） and decreased expression of IL-10 in the rat colon mucosa （P〈0.01）. Treatment with Chongyanqing decoction resulted in a significant reduction in DAI, CMDI, HS score and MPO activity （P〈0.01）, and decreased the expression oflCAM-1 （P〈0.01） and increased the expression oflL-10 （P〈0.01） in the colon mucosa. The expression oflCAM-1 in the colon mucosa was positively correlated to that oflL-10 （r=0.927, P〈0.01） and the activity of MPO （r=0.621, P〈0.01）. Conclusion Chongyanqing decoction has protective effect against rat ulcerative colitis, mediated probably by enhancement of IL-10 expression and reduction in ICAM-1 expression and neutrophil infiltration.

关 键 词：肠炎清 溃疡性结肠炎 细胞间的黏附分子-1 白介素-10 

分 类 号：R285.5[医药卫生—中药学]