硫辛酸对甲醛诱导小鼠肝细胞DPC形成的抑制作用  

Inhibitive Effects of Lipoic Acid on Formaldehyde Induced DNA-protein Crosslink Formation in Mice Liver

作　　者：梁思斯[1] 曹毅[1] 王昆[1] 付聪[1] 肖琳[1] 丁书茂[1] 袁均林[1] 杨旭[1] 

机构地区：[1]华中师范大学生命科学学院环境科学实验室,武汉430079

出　　处：《医学研究杂志》2008年第10期25-27,共3页Journal of Medical Research

基　　金：国家自然科学基金面上项目(30570799)

摘　　要：目的探讨甲醛所致氧化损伤与DNA-蛋白质交联关系。方法将昆明小鼠分3组:对照组,3.0mg/m3甲醛气体染毒组,3.0mg/m3甲醛气体染毒并注射α-硫辛酸(LA)组,处理后测定其肝脏谷胱甘肽(GSH)含量与DNA-蛋白质分子交联(DPC)系数。结果吸入3.0mg/m3甲醛气体组小鼠肝脏中GSH含量显著下降,DPC系数显著增大;吸入3.0mg/m3甲醛气体染毒并注射LA组小鼠GSH含量恢复到正常水平,DPC系数也恢复到正常水平。结论LA注射可抑制甲醛诱导小鼠肝细胞DPC的形成,DPC的产生与氧化损伤有一定的联系。Objective To explore the relation between oxidative damage and DNA-protein crosslink caused by formaldehyde. Methods Kun Ming mice were divided into 3 groups： control group, 3.0mg/m^3 gaseous formaldehyde inhaled group, 3.0mg/m^3 gaseous formaldehyde and lipoic acid （LA） treated group. The content of GSH and the amount of DPC were measured after exposure to formaldehyde. Results The content of GSH in liver cell decline significantly and the amount of DPC increased significantly after inhaled with 3.0mg/m^3 gaseous formaldehyde. The content of GSH and DPC were both recovered to control group after treated with 3.0mg/m^3 gaseous formaldehyde and LA. Conclusion LA injection could inhibit the formation of DPC in mice liver after exposure to formaldehyde. It is suggested that DPC is related with oxidative damage.

关 键 词：甲醛 氧化损伤 DNA-蛋白质交联(DPC) Α-硫辛酸 

分 类 号：R519[医药卫生—内科学] R977[医药卫生—临床医学]