血管内皮抑素转基因制剂的体外抗肿瘤作用及对大鼠大肠癌的影响  

The Antitumor Effects in vitro and Impact on Colon Cancer in Rats of Endostatin Transfected Bifidobacterium Oral Powder Preparation

作　　者：官成浓[1] 罗海清[1] 廖湘晖[1] 陈星明[1] 

机构地区：[1]广东医学院附属医院肿瘤诊治中心,湛江524001

出　　处：《医学研究杂志》2008年第10期64-66,共3页Journal of Medical Research

摘　　要：目的研究血管内皮抑素转基因双歧杆菌口服冻干粉剂(endostatin transfected bifidobacterium oral powder prepara-tion,ETB-2)体外抗肿瘤作用及对大鼠大肠癌的影响。方法采用MTT法检测ETB-2对人结肠癌细胞株LS-174T、人胃黏膜上皮细胞株GES-1的作用。以二甲肼(DMH)为诱发剂,观察ETB-2对大鼠大肠癌的防治作用;DMH诱癌及ETB-2灌胃时间为18周,在第33周结束实验。结果ETB-2在体外对人结肠癌细胞株具有显著的选择性杀伤作用,体内给药可明显降低大鼠大肠癌发生率(60%∶100%,P<0.01),但大肠癌平均个数及肿瘤体积指数的差异无显著性(P>0.05)。结论ETB-2对DMH诱导的大鼠大肠癌具有预防作用。Objective To study the antitumor effects in vitro and impact on colon cancer in rats of endostatin transfected bifidobacterium oral powder preparation （ ETB - 2）. Methods Growth inhibitory effect of the cecropins on normal human gastric epithelial cell line （GES- 1 ） and human colon adenoeareinoma cell line （LS - 174T） was observed using a microculturetetrazolium （MTT） colorimetric methods. Male Wistar rats were divided into 4 groups. All treatments were completed in a course of 18 weeks and the experiment was finished at week 33. Results The cecropins showed selective cytotoxic activity against the human colon adenocarcinoma cell line. There was a significant lower in incidence of colon tumors in rats （70% vs 100% ,P 〈0.01 ） ,but the tumor burden and tumor mass index were no difference between the groups（P 〉 0.05 ）. Conclusion ETB -2 has significant preventive effect on colon cancer induced by DMH in rats.

关 键 词：血管内皮抑素转基因双歧杆菌口服冻干粉刹(ETB-2) 大肠癌 二甲基肼 

分 类 号：R979.1[医药卫生—药品] R392-33[医药卫生—药学]