苯巴比妥钠诱导小鼠后血清与原代肝细胞培养物的生物学分析  

The Analysis of Mouse Blood Serum and Colture of Primary Hepatocytes Induced by Phenobarbital Sodiu

作　　者：伍通和[1] 陈南清[1] 黄永清[1] 陈爱静[1] 石艳芬[1] 

机构地区：[1]福建省龙岩市第二医院人工肝治疗中心,364000

出　　处：《医学研究杂志》2008年第10期72-73,75,共3页Journal of Medical Research

摘　　要：目的探讨经苯巴比妥钠(phenobarbital sodium,PBS)体内预诱导的成年动物肝细胞的生物学特征,研究效应肝细胞在生物人工肝(bioartificial liver,BL)与其他临床应用的潜在价值。方法12只成年雄性小白鼠随机分为预诱导组与对照组。预诱导组每日1次腹腔内注射PBS 45mg/kg,共7次;对照组注射NS。预诱导结束后两组小鼠做如下分析:检测血清总蛋白(TP)、尿功能素氮(BUN)、胆固醇(CHOL)、高密度脂蛋白胆固醇(HDLC)合成量;分离肝细胞培养48h后取培养液做同上4项生化分析;培养24h的肝细胞做MTT检测细胞增生活性;染色体显示法观察细胞分裂状况,以及肝细胞培养的存活期限和一般形态学观察。结果实验组血清TP与对照组有显著差异(t=2.678,P<0.5)。两组肝细胞培养液中TP、BUN、CHOL、HDLC含量差异无统计学意义。MTT实验两组的培养增生活性无显著差异。培养的生存期限均不超过72h,细胞一般形态未见变化差异。结论PBS预诱导可明显提高成年鼠血清TP含量和肝细胞的其他生物活性,有利于我们加深对PBS效应肝细胞在生物人工肝与其他临床应用潜能的探讨。Objective To investigate the biological characteritics of adult animal hepatocytes induced by phenobarbital sodium （PBS） in vivo,and to study the potential value of biological artificial liver of effective hepatocytes. Methods 12 adult male mice, are yandomly divided in to preinducing group and controll group. The preinducing group are intraperitoneally injected PBS per day, 45mg/kg for 7 times in total;the controll ware injected NS. after that, we detected the blood serum TP, BUN, CHOL, HDLC. And the same amount of isolative hepatocytes was developed after being developed 48h ; MTT was used to investigate the proliferation of hepatocytes after being developed 24h; chromosome was investigated to observe the cell division; and the survival deadline and morphology was also investigated. Resuits The TP had remarkable difference between the two groups（t = 2. 678,P 〈 0. 5）. Actirity was not found between two MTT groups, and the survival deadline did not surpass 72h,and the form of cell was normal. Conclusion PBS can raise the blood srem TP of adult mice significantly, and it allow us to study the use of PBS in basic and clinical research ,and study the potential value of effective hepatocytes.

关 键 词：苯巴比妥钠 预诱导 成年鼠 总蛋白 

分 类 号：R318.14[医药卫生—生物医学工程] R284.1[医药卫生—基础医学]