从细胞外信号调节激酶-1mRNA在肝纤维化大鼠肝组织中的表达来探讨姜黄素的治疗作用  

Expression of ERK-1mRNA in rat with liver fibrosis and the influence of curcumin

作　　者：胡泰洪[1] 徐敏[2] 朱锐[2] 刘建国[2] 杨玲[2] 

机构地区：[1]无锡市传染病医院中西医结合肝科,江苏无锡214005 [2]华中科技大学同济医学院附属协和医院

出　　处：《中西医结合肝病杂志》2008年第5期289-291,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：江苏省中医药管理局资助(No.H05096)

摘　　要：目的:探讨ERK-1mRNA在CCl4致实验性肝纤维化大鼠肝组织中的表达及姜黄素(curcumin)对它们的影响。方法:建立CCl4诱导的大鼠实验性肝纤维化的模型,用免疫组化法比较正常组、模型组以及姜黄素组大鼠的α-平滑肌肌动蛋白(α-smooth muscle action,α-SMA)的表达,用RT-PCR法比较各组大鼠肝组织胞外信号调节激酶-1(Extracellular-regulated kinase-1,ERK-1)的表达。结果:模型组大鼠α-SMA以及ERK-1mRNA的表达增高明显,而姜黄素组则较模型组低。结论:姜黄素可能通过抑制α-SMA的表达,抑制ERK-1的促肝星状细胞(hepatic stellate cell,HSC)的增殖作用,从而产生抗四氯化碳诱导的大鼠肝纤维化的作用。Objective： To evaluate Expression of ERK-1mRNA in rat with live,＇ fihrusis and the influence of cureumin to them. Methods： Liver fibrosis in rats were induced by carbon tetrachloride （CCl4） . Rats were divided into three groups： norreal control group, CCl4 model group and cureumin group. The expression of alpha-Smooth Muscle Action were detected by immunohistochemistry. The mRNA expression of extraeetlular signal-regulated kinase （ERK-1） were determined by RT- PCR. Results： The expression of alpha-Smooth Muscle Action were significantly h,wer in the rats trealed with eureumin compared with those of the model group （ P 〈0. 05） . The mRNA expression of ERK-1 were signitieantly higher in model group, but obviously lower in cureumin treated group （P 〈0.05 ） . Conclusion： cureumin is able to inhibit the aetiviation of HSC through suppress ing the expression of alpha-Smooth Muscle Action and it also can inhibit the proliferation of HSC through suppressing the mRNA expression of ERK-1.

关 键 词：姜黄素/药效学 细胞外信号调节激酶-1 肝纤维化 

分 类 号：R285.5[医药卫生—中药学]