核黄素合成途径关键酶:新型生防制剂筛选靶标  

Key Enzymes in Riboflavin Biosynthesis:Screening Targets of New Bio-control Agents

作　　者：王敏[1] 周德健[1] 裘娟萍[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,杭州310014

出　　处：《农药》2008年第11期790-793,813,共5页Agrochemicals

基　　金：973课题项目(2003CB114402)

摘　　要：植物和微生物可以自身合成核黄素,动物必须完全从食物中摄取,因此核黄素合成途径中关键酶抑制剂可开发为杀菌剂或除草剂,对人畜安全、无副毒作用。论述了有关核黄素合成途径中抑制作用靶标位点、筛选模型和抑制剂种类的研究进展,探讨了今后研究思路。以催化核黄素合成途径最后3步反应的羟基磷酸丁酮合成酶DHBPS、二氧四氢喋啶合成酶LS和核黄素合成酶RS作为抑制作用靶标位点,离体和活体筛选抑制剂。新筛选靶标和模型的建立有助于开发新型生防制剂。In contrast to animals, plants and microorganisms depend on the endogenous riboflavin by biologically synthesis. Therefore, the inhibitors against key enzymes in the riboflavin biosynthesis pathway can be developed into fungicides or herbicides which have non-toxic effects on people and livestock. Advances of the studies on the screening models for inhibitors against riboflavin biosynthesis are reviewed and the possible hot pot in the future studies on the bio-control agents are also discussed. Three enzymes, DHBP synthase, lumazine synthase （LS） and riboflavin synthase（RS） are respectively responsible for the last three kind of biological reactions during the riboflavin biosynthesis. With three enzymes DHBPS, LS and RS as targets, the compounds against the riboflavin synthesis in vitro and in vivo could be efficiently screened out. New screening targets and models contribute to the development of new bio-control agents.

关 键 词：核黄素合成 筛选靶标 生防制剂 酶抑制剂 筛选模型 

分 类 号：TQ450.6[化学工程—农药化工]