精脒促进E.coli非特异性转录的研究  

THE STUDY ON SPERMIDINE STIMULATING NONSPECIFIC TRANSCRIPTION IN E.COLI

作　　者：翟玲[1] 于成国[1] 

机构地区：[1]中国医科大学基础医学院免疫研究室

出　　处：《锦州医学院学报》1997年第6期8-10,共3页Journal of Jinzhou Medical College

摘　　要：本文以ＥｃｏＲＩ酶切的质粒ｐＢＲ３２２为模板，在体外转录系统中研究了精脒对Ｅ．ｃｏｌｉＲＮＡ聚合酶非特异性转录活性的作用。结果表明：０．５０—１０．００ｍＭ精脒对转录有明显促进作用，２．５０ｍＭ作用最强；精脒的对数浓度与转录产物相对放射活性间呈正态分布曲线。In this paper,the effect of spermidine on the nonspecific transcription activity of E.coli RNA polymerase was studied.We used EcoRI-digested plasmid pBR322 as the template in vitro.The results demonstrated that transcription was markedly stimulated by 0.50-10.00 mM spermidine among which 2.50 mM spermidine had the strongest effect,and the relation of logarithm concentrations of spermidine with the relative radioactivity of the transcriptions was normal distribution curve.The study suggested that the effect mechanism of spermidine on transcription might be dualistic or even be multidimensional.

关 键 词：精脒 转录 RNA聚合酶 多胺 免疫 

分 类 号：R392.13[医药卫生—免疫学] Q75[医药卫生—基础医学]