用RP-HPLC法测定人体内辛伐他汀血药浓度  

Determination of human plasma concentration of simvastatin by RP-HPLC method

作　　者：李娇娜 洪博[2] 赵春杰[2] 

机构地区：[1]辽宁中医药大学附属医院中西医结合分院,沈阳110101 [2]沈阳药科大学药学院药物分析教研室,沈阳110016

出　　处：《药学服务与研究》2008年第5期370-372,共3页Pharmaceutical Care and Research

摘　　要：目的：建立RP-HPLC法测定人血浆中辛伐他汀的含量。方法：采用Kromasil C18色谱柱（250mm×4．6mm，5μm）；流动相为甲醇-乙腈-水（80：5：15）；流速为1．0mL／min；检测波长238nm；柱温为30℃。结果：辛伐他汀的血药浓度在2．0～150．0ng／mL范围内线性良好，定量下限为2．0ng／mL。回归方程为A=1．589×10^8c-218．0，r=0．9999（n=7），低、中、高浓度（6．5、23．0、65．0ng／mL）的相对回收率分别为（100．28±3．35）％、（97．57±3．21）％和（100．25±2．82）％，日内和日间RSD为2．40％～4．32％（n=5）。结论：本法快速、高效、灵敏，适用于辛伐他汀的血药浓度测定。Objective： To establish a RP-HPLC method for determination of concentration of simvastatin in human plasma. Methods：Chromatography was carried out on a Kromasil C18 column, using methanol-aeetonitrile-water （80 ： 5 ： 15） as mobile phase at a flow rate of 1.0 mL/min. The detection wavelength was set at 238 nm. Column temperature was kept at 30 ℃ Results： The linearity range of simvastatin was 2.0-150.0 ng/mL and the quantitation limit was 2.0 ng/mL. The regressive equation was A=1. 589 × 10^3 c-218.0, r=0. 999 9 （n = 7）. The relative recovery rates of low, middle and high concentrations （6.5,23.0,65.0 ng/mL） were （100.28±3.35）%,（97.57±3.21）% and （100.25±2.82）%,respectively. Intra- and inter-day RSD was within 2.40%-4. 32% （n = 5）. Conclusion： The method is rapid, efficient, sensitive and suitable for determination of simvastatin in human plasma.

关 键 词：色谱法 高压液相 血药浓度 辛伐他汀 

分 类 号：R927.2[医药卫生—药学]