枳术汤对脾虚便秘小鼠胃动素和降钙素基因相关肽靶向调控的实验研究  

Empirical study of Zhi-zhu decoction for targeted regulation of motilin and calcitonin gene-related peptide in spleen asthenia type constipated mice

作　　者：郑学宝[1] 吴丹[1] 戴世学[1] 邹颖[1] 叶秋丽[1] 

机构地区：[1]广东医学院,湛江524023

出　　处：《中国药物与临床》2008年第11期869-872,921,共5页Chinese Remedies & Clinics

基　　金：广东省科技计划基金资助项目(2005B33001007)

摘　　要：目的观察枳术汤对脾虚便秘小鼠胃动素(motilin,MTL)和降钙素基因相关肽(calcitonin gene-related peptide,CGRP)含量的影响,探讨枳术汤对上述指标的靶向调控机制。方法采用番泻叶泄泻、饥饱失常以及限制饮水等复合因素造模方法建立脾虚便秘小鼠模型,造模组分别给予生理盐水、麻仁软胶囊、枳术汤治疗,用免疫组织化学方法检测胃窦、十二指肠、结肠MTL和CGRP变化。结果模型小鼠胃动素含量较空白组降低,而降钙素基因相关肽较空白组升高;治疗后枳术汤组胃动素含量较其他组升高,而降钙素基因相关肽较其他组降低,差异具有统计学意义(P<0.05)。结论枳术汤能使异常改变的胃动素和降钙素基因相关肽的含量恢复至正常水平,表明本方可能是通过对胃肠激素水平的影响而达到有效治疗脾虚滞结便秘的目的。Objective To observe the targeted regulation mechanism of Zhi-zhu Decoction on the content of motilin （MTL） and calcitonin gene-related peptide （CGRP） in spleen-deficiency constipated mice. Methods The mice models of spleen-deficiency constipation were established by exerting the factors of splenasthenic diarrhea, irregular diet and restricted fluid-consuming, and then treated with normal saline, MaRen soft-gel or Zhi-zhu Decoction. The contents of motilin and CGRP in the gastrointestinal tract were tested by immunohistochemical staining. Results The mice models had lower level of motilin and higher level of CGRP than those.on normal saline. After treatment, the Zhi-zhu Decoction group showed higher level of motilin but lower level of CGRP as compared with the other groups （P〈0.05）. Condnsion The Zhi-zhu Decoetion appeared potential to enhance the motilin and suppress the CGRP in spleen-deficiency constipation mice, which may be underlying its targeted regulation for the treatment of spleen-deficiency constipation.

关 键 词：脾虚 便秘 促胃动素 降钙素基因相关肽 枳术汤 

分 类 号：R285[医药卫生—中药学]