降脂益肝冲剂对非酒精性脂肪性肝炎大鼠肝脏PPARα mRNA表达的影响  

Effects of Jiang Zhi Yi Gan Granules on expression of hepatocyte peroxisome proliferator-activated receptors α in rats with nonalcoholic steatohepatitis

作　　者：殷果华[1] 赵和平[2] 

机构地区：[1]太原市第八人民医院药械科,030012 [2]山西医科大学第一医院综合科

出　　处：《中国药物与临床》2008年第11期873-875,共3页Chinese Remedies & Clinics

基　　金：山西省卫生厅科技攻关计划基金项目(200607)

摘　　要：目的观察降脂益肝冲剂对非酒精性脂肪性肝炎(NASH)大鼠肝脏过氧化物酶体增殖物激活受体α(PPARα)mRNA表达的影响,探讨其治疗NASH的机制。方法24只雄性Wistar大鼠随机分为3组:健康组普通饲料喂养,模型组和治疗组高脂饮食喂养。治疗组在高脂饮食13周末给予降脂益肝冲剂,同时模型组和健康组给予等量的生活饮用水灌胃,18周末处死各组大鼠。测定肝组织甘油三酯(TG)、总胆固醇(TC)、游离脂肪酸(FFA)含量及血清肿瘤坏死因子(TNF)-α水平,反转录聚合酶链反应(RT-PCR)法检测肝组织PPARα mRNA相对含量。结果模型组大鼠肝脏PPARα表达明显减少,肝脏TG、TC、FFA及血清TNF-α水平明显增加;治疗组肝脏PPARα表达明显增强,其余指标均明显下降。结论降脂益肝冲剂能显著促进PPARα的表达,可能是治疗NASH的分子机制之一。Objective To explore the effects and related mechanism of Jiang Zhi Yi Gan Granules on PPARα mRNA in liver of NASH rats. Methods Twenty-four male Wistar rats were randomized into 3 groups （n=8 each group） on standard diet （healthy group） or on high-fat diet （model group and treatment group）. After 13 weeks, Jiang Zhi Yi Gan Granules were given to the treatment group by garvage while the healthy and model groups received usual drinking water in the same way. All rats were sacrificed at 18 weeks. The expression of PPARα mRNA in NASH rat models induced by high fat diet was detected with RT-PCR. Meanwhile, the concentrations of TNF-α in serum and TG, TC and FFA in liver were detected. Results The model group showed significantly decreased hepatoeyte PPARα expression, increased serum TNF-α, liver TG, TC and FFA, while the treatment group showed increased hepatocyte PPARa expression and improvement of all the other biochemical parameters. Conclusion Jiang Zhi Yi Gan Granules increased the expression of PPARα mRNA in liver tissue of NASH rats, which may be part of mechanisms underlying its therapy for NASH.

关 键 词：脂肪肝 降脂益肝冲剂 过氧化物酶体增殖物激活受体 大鼠 

分 类 号：R285[医药卫生—中药学]