Cu(Ⅱ)-头孢曲松-赤藓红离子缔合物的UV-Vis、荧光和共振瑞利散射光谱分析及应用  

UV-Vis,Fluorescence and Resonance Rayleigh Scattering Spectra of Cu(Ⅱ)-Ceftriaxone-Erythrosin IonAssociation Complex and Their Analytical Application

作　　者：傅生会[1] 刘忠芳[1] 刘绍璞[1] 

机构地区：[1]西南大学化学化工学院,重庆400715

出　　处：《应用化学》2008年第11期1249-1254,共6页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(20475045)资助项目

摘　　要：在pH值为4.4~5.1的Britton-Robinson（BR）缓冲溶液中,头孢曲松钠（CTRX）与Cu（Ⅱ）及赤藓红（Ery）形成1∶2∶3的离子缔合物,可引起体系吸收光谱的变化、荧光猝灭和共振瑞利散射（RRS）的急剧增强,并产生了新的RRS光谱,最大RRS波长位于329 nm处。吸光度增加、荧光猝灭和RRS增强在一定CTRX浓度范围内成正比,三者的线性范围和检出限分别为4.4×10^-7-4.0×10^-6g/mL和1.3×10^-7g/mL（分光光度法）;2.4×10^-7~1.5×10^-6g/mL和7.2×10^-8g/mL（荧光猝灭法）及2.9×10-8~2.0×10^-6g/mL和8.8×10^-9g/mL（RRS法）。确定了适宜的测试条件,讨论了离子缔合物的组成和反应机理。考察了共存物质对RRS法测定CTRX的影响。结果表明,RRS法测CTRX不仅灵敏度高,而且选择性好,用于血清和尿样中头孢曲松钠的测定,取得满意结果。In a pH 4. 4 ~ 5. 1 Britton-Robinson（BR） buffer solution, ceftriaxone sodium（CTRX） reacts with Cu（ Ⅱ ） and Erythrosin（Ery） to form a 1：2：3 ion-association complex, which results in the change of absorption spectra, the quenching of fluorescence and the great enhancement of Resonance Rayleigh Scattering （RRS）, and the new RRS spectra appear. The maximum RRS wavelength is located at 329 nm. The increment of absorption, the quenching of fluorescence and the enhancement of RRS are all proportional to the CTRX concentration in a certain range. The quantitative determination ranges and the detection limits of the three methods for CTRX are 4.4×10^-7-4.0×10^-6g/mL and 1.3×10^-7g/mL（ spectrophotometry）, 2.4×10^-7~1.5×10^-6g/mL and 7.2×10^-8g/mL（fluorimetry）, and 2.9×10-8~2.0×10^-6g/mL and 8.8×10^-9g/mL（RRS） , respectively. The optimum condition of the experiment was investigated. The composition of ion-association complex and the mechanism of reaction were discussed. Besides, the effects of some co-existing substances on the determination of CTRX by RRS were investigated. The RRS method has not only high sensitivity, but also good selectivity. It can be applied to the determination of CTRX in serum and urine samples with satisfactory results.

关 键 词：头孢曲松钠 Cu(Ⅱ) 赤藓红 共振瑞利散射法 分光光度法 荧光猝灭法 

分 类 号：O655.1[理学—分析化学]