骨髓间充质干细胞支持脐血造血干／祖细胞体外扩增的研究  

In vitro expansion of hematopoietic stem/progenitor cells from human umbilical cord blood.

作　　者：高缨[1] 褚红女[1] 郭春娟[2] 沈美娟[1] 吕小玲[1] 侯宇宁 王金福[2] 

机构地区：[1]杭州师范大学附属医院杭州市第二人民医院妇产科,浙江杭州310015 [2]浙江大学细胞与遗传学研究所 [3]浙江省杭州市拱墅区小河湖墅社区卫生服务中心

出　　处：《中国医师杂志》2008年第10期1303-1306,共4页Journal of Chinese Physician

基　　金：浙江省教育厅基金资助项目（20061053）

摘　　要：目的体外分离培养人新鲜脐血（UCB）造血干细胞（HSC），利用骨髓间充质干细胞作为滋养层联合细胞因子组合以扩增脐血造血干／祖细胞。方法采用Ficoll淋巴细胞分离液密度梯度离心法分离UCBHSC，加入骨髓间充质干细胞和细胞因子，检测脐血造血干／祖细胞总有核细胞（NC）总数和CD34^＋细胞数。结果有核细胞总数扩增（75．2±15．0）倍，CD34^＋细胞数扩增（18．7±12．3）倍。结论体外HSC培养，加入骨髓间充质干细胞和细胞因子对造血干／祖细胞增殖有明显的作用。Objective To separate and cultivate homo umbilical cord blood （UCB） hematopoietic stem cell （HSC） in vitro, and utilize bone marrow desmohemoblast stem cell as trophoblastic layer combined with cytokine to amplify umbilical cord blood hematopoietic stem/progenitor cell. Methods Ficoll lymph-cell separating medium density gradient centrifugalization was used to segregate UCBHSC. Bone marrow desmohemoblast stem cell and cytokine were added, and the sum of NC cells and CD34 ^＋ cells was counted. Results The sum of NC cells amplified 75.2 ± 15.0 times, and the sum of CD 34 ^＋ cells amplified 18. 7 ± 12. 3 times. Conclusions It has significant effect on amplification of hematopoietic stem cell with bone marrow desmohemoblast stem cell and cytokine when HSC are cultured in vitro.

关 键 词：骨髓细胞/细胞学 间质干细胞/细胞学 细胞培养 造血干细胞 细胞增殖 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]