基因芯片技术检测大鼠心肌顿抑相关基因的差异表达  

Investigation of differential gene expression in rat myocardial stunning with cDNA microarry

作　　者：张燕[1] 英明中[1] 李小鹰[1] 韩为东[2] 任建平[3] 韩丽娜[1] 

机构地区：[1]解放军总医院老年心血管一科,北京100853 [2]解放军总医院分子生物室 [3]解放军总医院军事医学科学院放射与辐射医学研究所

出　　处：《中国医师杂志》2008年第10期1310-1313,共4页Journal of Chinese Physician

基　　金：国家自然科学基金项目（30640061）

摘　　要：目的研究大鼠心肌顿抑差异表达的基因，并进一步探讨心肌顿抑发生的机制。方法采用大鼠心肌顿押模型，取顿抑心肌组织及假手术组心肌组织标本，用27K Rat Genome Array进行全基因表达谱研究，筛选出差异表达的基因并进行功能分析。结果基因芯片检测发现了292个在顿抑心肌组织中2倍差异表达基因，其中上调199，下调93。表达上调的基因包括HSP70、Atf3、Gadd45b、Egr2、Verge等，上调最大幅度为23．1倍，主要涉及抗凋亡、血管和神经生长、转录和存活、修复等心脏保护性细胞因子和一些功能不明的基因，而下调基因多数功能不清。结论心肌顿抑通过上调一些基因的表达对缺血心肌起到保护作用，促进这些保护性基因的表达可能是将来缺血性心脏病的治疗目标。Objective To investigate the differential gene expression of myocardial stunning in rats, and provide basis for further studying the mechanism of myocardial stunning with cDNA microarry. Methods The model of myocardial stunning in rats was established. 27K Rat Genome Array was used to examine and analyze the differentially expressed genes of stunned myocardium tissues with the sham operated control myocardium tissues. Results There were 292 genes in stunned myocardium tissues with two folds differential expression, in which 199 genes were up- regulated and 93 genes were down-regulated. The up -regulated genes include HSPT0, Atf3, Gadd45b, Egr2, Verge＇and et al, and the highest up-regulation amplitude is 23.1 folds. Their function were involved in antiapoptosis, blood vessel and nerve growth, transcription, survival, recovery and et al. The function of most down-regulated gene were unknown. Conclusions Myocardial stunning may protect ischemic myocardium through up -regulating some cardioprotective genes. It may be a novel treatment target for ischemic myocardial disease in the future through improving the expression of these cardioprotective genes.

关 键 词：心肌顿抑/遗传学 寡核苷酸序列分析 基因 基因表达 

分 类 号：R541[医药卫生—心血管疾病]