血管内皮细胞生长因子对兔异体重建后交叉韧带血管再生的影响  

Effect of vascular endothelial growth factor on vascular regeneration of reconstructed posterior cruciate ligament allograft transplantation in rabbits

作　　者：孙英华[1] 王莉[1] 焦兆德[1] 

机构地区：[1]潍坊医学院第三附属医院骨外科,山东省潍坊市262500

出　　处：《中国组织工程研究与临床康复》2008年第42期8225-8229,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:膝关节后交叉韧带重建的研究目前大多集中在手术技巧和移植物材料的选择上,而对重建后移植物血管再生情况的相关报道不多,尤其对异体移植物血管再生情况的相关研究更少。目的:拟观察应用血管内皮细胞生长因子对兔异体重建后交叉韧带血管再生的影响。设计、时间及地点:析因设计,于2006-03/2007-09在潍坊医学院第三附属医院骨科实验室完成。材料:68只成年雌性日本大白兔,体质量(3.3±0.1)kg,其中23只用于切取股骨-前交叉韧带-胫骨复合体以建立兔后交叉韧带异体重建动物模型。方法:45只兔随机分为3组,每组15只。对照组不作任何处理;磷酸盐缓冲液组向患膝关节腔内注入0.2mL磷酸盐缓冲液;血管内皮细胞生长因子组向患膝关节腔内注入30μg血管内皮细胞生长因子(溶于0.2mL磷酸盐缓冲液)。主要观察指标:①重建后移植物的免疫排斥情况。②第3,6,12周每组各随机选5只兔取标本作免疫组织化学染色,Chalkley计数法对移植物韧带部分的中1/3处微血管密度计数。结果:45只兔均进入结果分析。每只兔切开关节后未见积液及软骨破坏,移植物大体形态接近正常后交叉韧带,无变性坏死及融解脱落等免疫排斥表现。血管内皮细胞生长因子组实验兔关节内可见大块血运丰富的组织,而对照组和磷酸盐缓冲液组没有。3组各时间点移植物微血管密度相比,血管内皮细胞生长因子组高于对照组和磷酸盐缓冲液组(P<0.01);第6周高于第3周和第12周(P<0.01),第12周低于第3周(P<0.05);微血管密度计数中组别与时间无交互效应。结论:局部应用血管内皮细胞生长因子对兔异体移植重建后交叉韧带移植物血管再生具有明显促进作用。BACKGROUND： Currently, the research of posterior cruciate ligament （PCL） construction mainly focuses on the surgical technique or graft selection. However, studies on vascular regeneration after construction are few, especially those on allograft construction. OBJECTIVE： To observe the effect of vascular endothelial growth factor （VEGF） on vascular regeneration of reconstructed PCL with femur-anterior cruciate ligament （ACL）-tibia allograft in rabbits. DESIGN, TIME AND SETTING： The factorial design experiment was performed at the Orthopedics Laboratory of the Third Affiliated Hospital of Weifang Medical College from March 2006 to September 2007. MATERIALS： Sixty-eight adult female Japanese white rabbits, weighing （3.3_＋0.1） kg, were adopted. Twenty-three of them were used to excide the femur-ACL-tibia complexes to establish the animal model of PCL reconstruction with allografi. METHODS： Forty-five rabbits were randomly divided into 3 groups, 15 rabbits in each group. In the control group, no additional treatments were applied. In the phosphate-buffered saline （PBS） group, 0.2 mL of PBS was injected into the knee joint. In the VEGF group, 30 μ g VEGF mixed with 0.2 mL PBS was injected into the knee joint. MAIN OUTCOME MEASURES： The immunological rejection of allograft was observed after reconstruction. Five rabbits were randomly selected in each group at 3, 6, 12 weeks, respectively, for immunohistochemistry staining; and the microvessel density of the medio-one-third ligament part of allograft was evaluated with the Chalkley scoring method. RESULTS： Forty-five rabbits were involved in the result analysis. There were no hydrops articuli or aneretic cartilage when every rabbit＇s knee joint was opened. The gross morphology of the grafts was similar to normal posterior cruciate ligament. Immunological rejection, such as degeneration, necrosis, dilapsus or defluxion was not appeared. Plenty of good vascular tissue in the arthrosis could be seen in the VEGF group, but could not he

关 键 词：血管内皮细胞生长因子 重建 后交叉韧带 血管再生 

分 类 号：R392[医药卫生—免疫学]