扰动剪切流场对血管内皮细胞小窝蛋白再分布和表达量的影响  

Disturbed shear stress affects disposition of caveolin-1 in endothelial cells

作　　者：刘冰[1] 蔡绍皙[1] 张莉[1] 

机构地区：[1]重庆大学生物工程学院,400044

出　　处：《中华心血管病杂志》2008年第10期927-930,共4页Chinese Journal of Cardiology

基　　金：国家自然科学基金资助项目（10272122）

摘　　要：目的利用能模拟动脉粥样硬化好发部位模型的流动腔来探讨扰动剪切力对内皮细胞膜信号传导中介——小窝蛋白分布和表达量的影响。方法将细胞接种到流动腔，取加载作用6、12、24和48h后的细胞与静止态的细胞进行活细胞染色，利用激光共聚焦显微镜扫描，分析内皮小窝蛋白的分布情况，并在相同时间点取加载作用的细胞与静止态的细胞进行RealTimePCR，检测内皮小窝蛋白相对表达量的变化。结果扰动剪切作用下，小窝蛋白发生明显的再分布，向细胞边缘聚集，并没有出现单边极化的现象。内皮细胞小窝蛋白-1相对表达量随着加载时间的延长逐渐减少，在扰动剪切力作用下48h后内皮细胞小窝蛋白-1相对表达量减少了约40％。结论扰动流影响了小窝蛋白在细胞中的再分布和相对表达量，可能进一步通过力-化学信号的耦合形式，最终对细胞的生物学行为产生影响。Objective To observe the distribution and expression changes of caveolin-1 under shear stress in a turbulence flow channel model mimicking susceptible sites of atherosclerosis in vitro. Methods Human umbilical vein endothelial cells （HUVECs） were inoculated to flow channel and then exposed to disturbed shear stress for 6, 12, 24 and 48 hours, respectively. HUVECs inoculated to flow channel without shear stress served as controls. Immunofluorescence staining was performed with SABC-FITC to detect the caveolin-1 distribution and RT-PCR was used to determine the mRNA expression of caveolin-1 gene in these ceils. Results Confocal microcopy revealed ellipse-like shaped HUVECs under disturbed shear stress and caveolin-1 didn＇t move toward to the upstream edge of HUVECs but moved toward to the edge of HUVECs, and expression of caveolin-1 gene in HUVECs was significantly downregulated after exposure to shear stress for 48 hours compared to HUVECs without shear stress stimulation. Conclusion Disturbed shear stress affected disposition and expression of caveolin-1 which might be responsible for shear stress induced endothelial cell pathological atherosclerotic changes.

关 键 词：扰动剪切力 小窝蛋白-1 内皮细胞 实时定量PCR 

分 类 号：R686[医药卫生—骨科学]