微电极阵芯片技术在整体心脏、心肌组织片和培养心肌中的应用研究  

Cardiac field potentials and activation sequence in Langendorff perfused heart, cardiac tissue slices and cultured ventricular myocytes recorded by microelectrode arrays system

作　　者：侯月梅[1] 娜几娜[1] 热依兰·艾沙 

机构地区：[1]新疆医科大学第一附属医院心脏中心心功能、心律失常病区,乌鲁木齐830054 [2]新疆库车县维吾尔医院

出　　处：《中华心血管病杂志》2008年第10期944-946,共3页Chinese Journal of Cardiology

基　　金：国家自然科学基金资助（30360033）

摘　　要：目的探讨微电极阵芯片技术（microelectrode arrays，MEA）在整体心脏、心肌组织片和培养心肌电活动的应用。方法用MEA技术记录豚鼠整体心脏、SD大鼠心脏组织片和小鼠乳鼠心脏培养心肌的组织场电位变化规律。结果（1）豚鼠整体心脏灌流30～90min，记录到稳定的自主节律（120±90）次／min；心室和心房肌的场电位时程为（210±78）ms和（164±58）ms，房室传导时间为（320±150）ms。（2）SD大鼠心脏组织片兴奋性可以保持2h，心室和心房组织的场电位时程为（115．80±11．61）ms和（83．71±6．48）ms。（3）小鼠乳鼠心脏原代培养自发搏动频率为（150±100）次／min，培养2—72h，记录到同步的多位点场电位，并能随刺激频率同步显示场电位变化。结论MEA技术是一个敏感、低噪音和长时间稳定的记录整体动物心脏、心脏靶点取样组织和培养心肌细胞的场电位、细胞间电活动传导和激动起源规律的研究工具。Objective To observe field potentials （FPs） and activation sequence at Langendorff perfused guinea pigs heart, SD rat cardiac tissue strips perfused by Tyrode＇s solution and cultured ventricular cardiomyocytes of suckling mice by microeletrode arrays （MEA） technique. Method FPs and activation sequence were recorded from perfused heart, cardiac tissue strips （5 mm× 5mm） and cultured ventricular cardiomyocytes by MEA. Results （ 1 ） FPs could be recorded in hearts perfused for 30 to 90 rain with a heart rate 90 - 120 beats/rain. FP durations of both ventricular and atrial tissue were （210 ± 78 ） ms and （164 ±58） ms, respectively and atrial ventricular conduction time was （320 ± 150）ms. （2） The excitability of Tyrode＇s solution perfused tissue strips was visible for more than 2 h, and FP durations of ventricular and atrial tissue strips were （ 115.80 ±11.61 ） ms and （ 83.71 ± 6. 48 ） ms, respectively. （ 3 ） Spontaneous beating frequency （ 150 ± 100） beats/min and FPs could be readily recorded in cardiomyocytes cultured between 2 to 72 hours. Conclusion MEAs is a sensitive, low noise and stable technique for observing local tissue action potential and activation sequence of whole heart, cardiac tissue strips and cultured cardiomyocytes.

关 键 词：微电极 心脏 心肌 细胞培养 

分 类 号：R686[医药卫生—骨科学]