电针双向调节白细胞数量的作用与脾血窦基膜小孔活动的关系  

Relationship Between Electroacupuncture-induced Regulatory Effect on Leukocytes and the Caliber of Splenic Sinusoid Basal Lamina Eyehole on Rabbits

作　　者：毛慧娟[1] 吴会会[1] 卜林凌[1] 周玉宝[1] 孙佳[1] 孙平龙[1] 

机构地区：[1]上海中医药大学针灸推拿学院,上海201203

出　　处：《针刺研究》2008年第5期291-295,共5页Acupuncture Research

基　　金：国家中医药管理局重点学科<针灸学>资助项目;上海市重点学科建设资助项目(No.T0302)

摘　　要：目的:探讨脾脏血窦基膜小孔参与电针双向调节外周血白细胞的过程。方法:实验家兔75只,随机分成5组:白增治疗组、白增对照组、白减治疗组、白减对照组和正常对照组,每组15只。家兔耳缘静脉分别注射大肠杆菌菌液和环磷酰胺,复制白细胞增多症模型和白细胞减少症模型,每天耳缘静脉采血,测外周血白细胞计数。治疗取"大椎"、双侧"足三里"、双侧"膈俞",电针20 min,每天1次,共5 d。治疗结束后,切取脾脏,制作脾脏的电镜标本,扫描电镜下观察脾血窦基膜小孔,显微图像分析软件计算脾血窦基膜小孔口径面积。结果:两治疗组与各自模型对照组比较,白细胞数恢复较快(P<0.05,P<0.01);白减治疗组较白减对照组脾血窦基膜小孔口径增大(P<0.05);白增治疗组较白增对照组脾血窦基膜小孔口径缩小(P<0.05)。结论:外周血白细胞数量降低或升高时,电针可以调整白细胞数量趋向正常水平,该作用与脾脏血窦基膜小孔相应的增大或缩小有关。Objective To study the underlining mechanism of electroacupuncture （EA） in regulating leukocyte counts in the rabbit. Methods A total of 75 rabbits were randomly divided into normal control, leukocytosis model, leukocytosis＋ EA, leukopenia model, leukopenia＋ EA groups, with 15 cases in each. EA （2-20 Hz, 1-2 mA） was applied to ＂Dazhui＂ （DU 14）, bilateral ＂Zusanli＂ （ST 36） and bilateral ＂Geshu＂ （BL 17） for 20 min, once daily for 5 days. Leukocytosis and leukopenia models were duplicated by intravenous injection of coliform （0. 1 ml/kg） and cyclophosphamide （30 mg/kg） separately. Peripheral blood leukocyte counts were detected every day. At the end of the experiment, the rabbit＇s spleen tissue was collected under anesthesia, and the diameter of splenic sinusoid basal lamina eyehole was measured under scanning electronic microscope. Results Compared with normal control group, after i.v. coliform and cyclophosphamide, leukocytes increased and decreased significantly and respectively, while the area of the splenic sinusoid basal lamina eyehole in leukocytosis model group increased lightly and that of leukopenia model group reduced obviously （P〈0.01）. In comparison with their individual model group, the leukocyte count in leukocytosis＋ EA group decreased considerably （P 〈 0.05）, while that of leukopenia ＋ EA group increased markedly （P〈0.01）. The area of splenic sinusoid basal lamina eyehole in leukocytosis＋ EA group decreased apparently （P〈0.05）, and that in leukopenia＋ EA group increased remarkably （ P〈0.05）. Conclusion EA is able to regulate the leukocyte counts in coliform-induced leukocytosis and cyclophosphamide-induced leukopenia rabbits, which may be related with its effects in improving the splenic sinusoid basal lamina eyehole activity.

关 键 词：白细胞增多 白细胞减少 电针 双向调整 脾血窦基膜小孔 

分 类 号：R542.22[医药卫生—心血管疾病] R289.5[医药卫生—内科学]