手针与电针“足三里”穴镇痛效应的穴位传入机制差异性分析  

Analysis on the Difference of Afferent Mechanism of Analgesic Signals from Manual Acupuncture and Electroacupuncture of "Zusanli"(ST 36)

作　　者：余晓佳[1] 詹睿 黄红[1] 丁光宏[1,2] 

机构地区：[1]复旦大学力学与工程科学系,上海200433 [2]上海市针灸经络研究中心,上海201203

出　　处：《针刺研究》2008年第5期310-315,共6页Acupuncture Research

基　　金：国家“973”项目(2005CB523306);上海市科技发展基金(05DZ19747);上海市教委重点学科(T0302)资助

摘　　要：目的:观察手针与电针"足三里"穴位镇痛效应的穴位传入机制的差异性,分析这两种临床常用针刺方法的穴位始动信号的启动机制。方法:SD成年大鼠64只,随机分为正常组、模型组、手针组、电针组、胶原酶手针组、胶原酶电针组、色甘酸钠手针组和色甘酸钠电针组,每组8只。采用踝关节腔内注射完全弗氏佐剂(0.05 ml)建立大鼠佐剂性关节炎(AA)模型。在AA大鼠模型上,胶原酶手针和电针组于针刺前30 min在患侧"足三里"注射Ⅰ型胶原酶20μl(5 mg/ml)破坏局部组织胶原纤维,色甘酸钠手针和电针组于针刺前30 min在患侧"足三里"注射色甘酸钠20μl(0.02 g/ml)对局部肥大细胞进行功能屏蔽,并予以手针或电针。以大鼠缩爪反射潜伏期、痛阈变化率和肥大细胞脱颗粒率为镇痛效果和穴位激活的观察指标,比较穴区组织中胶原纤维对手针或电针"足三里"的镇痛效应及肥大细胞功能的影响。结果:Ⅰ型胶原酶或色甘酸钠"足三里"预处理后手针镇痛效果明显被削弱(P<0.05),而电针镇痛效果未受影响(P>0.05);两种预处理方法下,手针或电针穴位肥大细胞脱颗粒率均受到明显抑制(P<0.05)。结论:手针的针刺穴位始动信号可能是由胶原纤维参与介导的,并通过激活肥大细胞的功能,将有效信息经外周传入到中枢;而电针的针刺信号可能是直接激活外周神经感受器,由神经介导的。Objective To observe the difference of signaling afferent mechanism of acupoints analgesic effect between manual acupuncture （MA） and electroacupuncture （EA） of ＂Zusanli＂ （ST 36） in order to analyze the initiating mechanism of these two commonly used clinic acupuncture methods. Methods A total of 64 SD rats were randomized into control, model, MA, EA, collagenase（type- Ⅰ ）-pretreatment （OP） ＋ MA, OP ＋ EA, disodium cromoglicate-pretreatment （DSCP） ＋ MA and DSOP ＋ EA groups with 8 cases in each. Arthritis model was established by intra-joint injection of complete Freunds＇ agent. MA（rotation, 20 min）, and EA （21 min） were applied to bilateral ＂Zusanli＂ （ST 36） respectively in different groups. Pain threshold （PT, paw withdraw latency） was detected with 336 GT PAW/TAIL STIMULATOR and degranulated mast cells were counted under microscope separately. Results Compared with normal control group, PT values of model, MA, EA, CP＋ MA, CP＋ EA, DSCP＋ MA and DSCP＋ EA groups lowered significantly before treatment （P〈0.05）. Comparison of the same one group showed that after the treatment, PT values of MA and EA groups increased considerably （P〈0.05）. PT changing rates of CP＋ MA and DSCP ＋ MA were significantly lower than that of MA group （P〈0.05）, suggesting disappearance of MA analgesia （not EA analgesia） after lesion of collagen protein by CP in ST36 area. In comparison with control group, the degranulation ratios （DGR） of mast cells in MA and EA groups increased significantly （P〈0.05） ; while compared with MA and EA groups separately, DGR of the corresponding OP ＋ MA and DSOP ＋ MA, and CP ＋ EA and DSGP ＋ EA groups decreased markedly （P〈0.05）, displaying an inhibition of mast cell degranulation following OP and DSCP in ST36 area. Conclusion Both MA and EA can induce analgesia and concomitant increase of DGR of mast cells. The collagen fibers may contribute to MA initiated analgesic effect while the peripheral ne

关 键 词：佐剂性关节炎 针刺镇痛 胶原纤维 肥大细胞 

分 类 号：R245.31[医药卫生—针灸推拿学]