日本血吸虫TCTP编码基因DNA免疫的效果评价  

Immuno-Responses of Genetic Immunization of BALB / c Mice with Recombinant Plasmid pEGFP Containing TCTP Gene of Schistosoma japonicum

作　　者：阮志燕[1,2] 吴忠道[1] 曹爱莲[1] 

机构地区：[1]中山大学中山医学院寄生虫学教研室,广州510080 [2]中国科学院广州生物医药与健康研究院,广州510663

出　　处：《热带医学杂志》2008年第10期1009-1011,1015,共4页Journal of Tropical Medicine

基　　金：国家自然科学基金(No.30070683)

摘　　要：目的评价pEGFP-TCTP真核重组质粒联合免疫佐剂CpG诱导小鼠保护性免疫的效果。方法构建日本血吸虫TCTP真核重组质粒,将pEGFP-TCTP注射入BABL/c小鼠,采用ELISA法检测免疫小鼠血清中IFN-γ水平;尾蚴攻击感染后45d处死小鼠,收集成虫和虫卵,计算减虫率和减卵率;小鼠肝脏送病理切片,计算虫卵肉芽肿的数量。结果TCTPDNA免疫保护性效果显示:实验组与阴性对照组相比,显示出一定的保护性效果,TCTP组的减虫率和减卵率分别为43.91%和53.48%,与GST组效果相似。DNA免疫后及感染后期,TCTP组与PBS对照组血清IFN-γ水平有显著性差异(P<0.05);攻击感染前后组间比较发现,GST组和GST+CpG组有显著性差异(P<0.05),提示CpG显著提高了DNA免疫的效果。结论SjTCTPDNA免疫能诱导较明显的保护性Th1免疫应答,CpG基序可以有效的诱导保护性Th1免疫应答,是一种有效的DNA免疫佐剂。Objective To study the protection effect of genetic immunization with the translationally controlled tumor protein （TCTP）and CpG as adjuvant against Schistosoma japonicum. Method BALB/c mice were inoculated with plasmid DNA and adjuvant CpG twice. The mice were challenged with cercariae of Schistosoma japonicum. Adult worms and eggs were collected after challenge. The protective effect was evaluated by calculating the worm and the egg reduction rate. Result The serum levels of IFN-γ of the TCTP group after immunization and after challenge were significantly higher that those of the PBS control group （P〈0.01）. This difference was also significant at the late stage of infection （P〈0.05）. The worm reduction rate and egg reduction rate of the TCTP group were 43.91% and 53.48%, respectively, which were similar to the GST group. Conclusion The genetic immunization with pEGFP-TCTP induced primary Thl immune response, and give a reasonable protection against S. japonicum infection.

关 键 词：日本血吸虫 翻译控制肿瘤蛋白(TCTP) CPG基序 DNA免疫 

分 类 号：R383.24[医药卫生—医学寄生虫学]