喜树生物碱在喜树植株中的分布  

Distribution of Camptothecin and 10-hydroxycamptothecin in Plant of Camptotheca acuminata Decne

作　　者：杨磊[1] 李晓娟[1] 赵春建[1] 李佳慧[1] 祖元刚[1] 

机构地区：[1]东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040

出　　处：《植物生理学通讯》2008年第5期873-876,共4页Plant Physiology Communications

基　　金：国家科技支撑计划项目(2006BAD18B04)

摘　　要：采用高效液相法测定喜树植株不同部位的生物碱(喜树碱、10-羟基喜树碱)含量的结果表明,十年生喜树叶中的喜树碱含量最高(0.1102%),非木质化茎的(0.0588%)略高于木质化茎的(0.0491%),主干上部(0.0604%)和主干近根部(0.0641%)高于主干中部(0.0447%),根部一级侧根(0.0968%)中含量也较高。10-羟基喜树碱在二级侧根中含量最高(0.4950%),木质化茎(0.0473%)明显高于非木质化茎(0.0125%),主干中含量分布与喜树碱相似,叶中含量为0.0905%。Contents of two main alkaloids [camptothecin （CPT） and 10-hydroxycamptothecin （HCPT）] in plant of Camptotheca acuminata were detected by HPLC. The results showed that CPT content in leaves of 10 years old C. acuminata was the highest （0.1102%）, CPT content in non-lignified stem （0.0588%） was higher than that in lignified stem （0.0491%）, and CPT content in the middle trunk was lower than those in the other parts. And also CPT content in first lateral roots （0.0968%） was higher. HCPT content in the sub lateral roots was the highest （0.4950%）. And HCPT content in the lignified stem （0.0473%） was higher than that in non-lignified stem （0.0125%） obviously. The distribution of HCPT in the trunk was the same as CPT. HCPT content in leaves was 0.0905%.

关 键 词：喜树 10-羟基喜树碱 喜树碱 高效液相色谱 

分 类 号：Q946[生物学—植物学]