麻疯树两种核糖体失活蛋白的表达模式分析  

Analysis on the Expression Patterns of Two Ribosome Inactivating Proteins from Jatropha curcas L.

作　　者：秦小波[1] 邵彩霞[1] 徐莺[1] 陈放[1] 

机构地区：[1]四川大学生命科学学院,成都610064

出　　处：《植物生理学通讯》2008年第5期891-896,共6页Plant Physiology Communications

基　　金：国家自然科学基金(30670204);国际科学技术合作项目(2004DFB00300;2006DFB63400);教育部科技重点项目(104151)

摘　　要：目前,麻疯树核糖体失活蛋白已发现2种:种子中的毒蛋白curcin和叶片中的逆境诱导蛋白curcin-L,其基因的表达模式均呈现出器官特异性。mRNA和蛋白质水平分析显示,curcin只在种子的胚乳中积累,且表达开始于心型胚时期,成熟时达到最高;而curcin-L在正常生理条件下并不表达,但受脱落酸、水杨酸、干旱、高低温等胁迫诱导时仅在叶片中表达。So far, two types of ribosome inactivating proteins （RIPs） have been found in Jatropha curcas, one is curcin in seeds, and the other is curcin-L in leaves. The results showed that the character of their genes＇ expression pattern was organ-specific. Analysis on the levels of mRNAs and proteins showed that, curcin only expressed and accumulated in endosperm of Jatropha curcas from heart-shape embryo period to mature embryo period. Curcin-L didn＇t express under common physiological conditions. However, it could be induced in leaves by abscisic acid, salicylic acid, drought, high or low temperature and so on.

关 键 词：麻疯树 核糖体失活蛋白 胁迫 表达模式 

分 类 号：Q943[生物学—植物学]