Influence of Formate on Bioactivity Material-thuringiensin Synthesized by Bacillus thuringiensis YBT-032  

作　　者：王志 喻子牛 

机构地区：[1]College of Bioengineering, Hubei University of Technology [2]State Key Laboratoy of Agricultural Microbiology National Engineering Research Center for Microbial Posticides, Huazhong Agricultural University

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2008年第5期691-693,共3页武汉理工大学学报（材料科学英文版）

基　　金：the National Natural Science Foundation of China (No.30170032);Affiliated institutions 1 and 2 contributed equally to this work

摘　　要：The biological method to synthesize thuringiensin and the influence of formate on thuringiensin biosynthesis were investigated. Addition of 1.00 g/L formate to growth medium of bacillus thuringiensis YBT-032 resulted in significant enhancements in productions of citrate, α -ketoglutarate, intracellular adenine and thuringiensin. These results demonstrate that added formate attends metabolism of cell, facilitates carbon metabolic flux in tricarboxylic acid cycle and hexose monophosphate pathway. As a carbon source, formate facilitates cell growth, increases glucose consumption and enhances the ability of cell to synthesis adenine analogues, and subsequently thuringiensin. Thuringiensin production rate significantly enhanced from 6.44 to 8.46 mg·g^-1 · h and transformation ratio from glucose to thuringiensin increased by 43.30%.The biological method to synthesize thuringiensin and the influence of formate on thuringiensin biosynthesis were investigated. Addition of 1.00 g/L formate to growth medium of bacillus thuringiensis YBT-032 resulted in significant enhancements in productions of citrate, α -ketoglutarate, intracellular adenine and thuringiensin. These results demonstrate that added formate attends metabolism of cell, facilitates carbon metabolic flux in tricarboxylic acid cycle and hexose monophosphate pathway. As a carbon source, formate facilitates cell growth, increases glucose consumption and enhances the ability of cell to synthesis adenine analogues, and subsequently thuringiensin. Thuringiensin production rate significantly enhanced from 6.44 to 8.46 mg·g^-1 · h and transformation ratio from glucose to thuringiensin increased by 43.30%.

关 键 词：bacillus thuringiensis PRECURSOR FORMATE thuringiensin 

分 类 号：TB39[一般工业技术—材料科学与工程]