MADS-box基因cDNA片段在甜菜染色体上的定位  

Chromosomal localization of MADS-box gene cDNA in sugar beet

作　　者：王玉婷[1] 高庆和[2] 马春泉[1] 王冰[1] 汪立法[1] 曹洪祥[1] 杜萍[1] 张喜波[1] 李海英[1] 

机构地区：[1]黑龙江大学生命科学学院分子生物学重点实验室,哈尔滨150080 [2]黑龙江省肇东市农业技术推广中心,肇东151100

出　　处：《黑龙江大学自然科学学报》2008年第5期648-651,655,共5页Journal of Natural Science of Heilongjiang University

基　　金：国家自然科学基金资助项目(30871566);黑龙江省自然科学基金资助项目(C2007-37);黑龙江大学青年基金资助项目

摘　　要：以甜菜MADS-box基因cDNA片段为探针,采用Southern杂交证实了其在甜菜基因组中为低拷贝序列;利用荧光原位杂交技术(FISH)将其初步定位在二倍体栽培甜菜(Beta vulgar-is)A2Y的第2号染色体上。研究成功地实现了小片段低拷贝的功能基因在甜菜染色体上的定位,为今后甜菜遗传物理图谱的构建提供了有效可行的方法。The cDNA of MADS - box gene as the probe was used to study the properties, and the results of Southern analysis indicated that it had low copies in the genome of sugar beet and the gene was located on diploid Beta vulgaris A2Y chromosome No. 2 by the method of fluorescence in situ hybridization （FISH）. The localization of low copy gene on the chromosome of sugar beet was firstly achieved successfully. It may provide effective method for the construction of physical map in sugar beet.

关 键 词：MADS-BOX基因 甜菜 荧光原位杂交(FISH) 定位 

分 类 号：S566.3[农业科学—作物学]