中国小麦条锈菌主要小种和流行菌系的AFLP分析  

AFLP analysis of major races and epidemic strains of Puccinia striiformis f.sp.tritici in China

作　　者：郝保军[1] 张勃[1] 王保通[1] 李强[1] 李高宝[1] 王芳[1] 康振生[1] 

机构地区：[1]西北农林科技大学植保学院与陕西省农业分子生物学重点实验室,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2008年第11期71-76,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"十一五"科技支撑计划项目(2006BAD08A05);陕西省"13115"科技创新工程重大科技专项(2006K02-G10-01);教育部长江学者和创新团队发展计划项目(200558);高等学校学科创新引智计划项目(No.B07049)

摘　　要：【目的】明确近年来我国小麦条锈菌主要小种及流行菌系的分子遗传关系。【方法】利用AFLP技术,对我国近年来小麦条锈菌(Puccinia striiformisf.sp.tritici)的主要流行菌系,特别是水源11类群进行了DNA指纹分析,并与毒性分析结果进行了比较。【结果】(1)小麦条锈菌流行菌系之间,无论是毒性特征还是DNA指纹特征,均存在丰富的遗传变异,但二者并不相关;(2)AFLP聚类分析显示,水源11类群的不同类型之间并没有很近的亲缘关系,其在遗传上不属于同一个来源。【结论】新出现的致病类型很可能由较近的共同起源菌系进化而来;AFLP技术非常适合于小麦条锈菌的遗传分析,能客观揭示小种(菌系)之间的亲缘关系。[Objective] The study clarified the molecular genetic relations of major races and epidemic strains of Puccinia striiformis f. sp. tritici in recent years in China. [Method] Amplified fragment length polymorphisim（AFLP） was used to fingerprint the dominant epidemic strains in reneent years,especially, Shuiyuanll group of Puccinia striiformis f. sp. tritici,the pathogen of wheat stripe rust,compared to the result of virulence characterisc of pathogen. [Result] （1）genetic diversity of both virulence and fingerprint characteristic existed in the pathogen populations, but there was not significant correlation between the two characteristics;（2）according to AFLP cluster analysis, different pathotypes in shuiyuan11 group didn＇t share close evolutionary relationships and they evolved independently. [Conclusion] Thus the new pathotypes might be developed from those earlier strains with the same origin and AFLP is very suitable for genetic analysis of pathogens and it can objectively reveal evolutionary relationships among the different races.

关 键 词：小麦条锈菌 毒性分析 AFLP 聚类分析 

分 类 号：S435.121.42[农业科学—农业昆虫与害虫防治]