香果树体细胞胚胎发生过程中4种同工酶的研究  

Studies on Four Kinds of Isozymes during Somatic Embryogenesis of Emmenopterys henryi Oliv.

作　　者：熊丹[1] 陈发菊[2,1] 梁宏伟[1] 王玉兵[1] 李晓玲[1] 

机构地区：[1]三峡大学生物技术中心,湖北宜昌443002 [2]湖北民族学院湖北省生物资源保护与利用重点实验室,湖北恩施445000

出　　处：《西北植物学报》2008年第10期2011-2016,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：湖北省生物资源保护与利用重点实验室开放基金(2007010);国家林业局自然保护区研究中心项目(460-8101);宜昌市科技重点攻关项目(A06209)

摘　　要：用非变性聚丙烯凝胶电泳技术对香果树体细胞胚胎发生及形态建成过程中过氧化物酶(POD)、酯酶(EST)、淀粉酶(AMY)和超氧化物歧化酶(SOD)4种同工酶进行分析。结果表明:香果树体细胞胚胎发生及形态建成过程中,POD、EST、AMY和SOD活性变化与胚性愈伤组织的诱导及体细胞胚的发生发育密切相关。非胚性愈伤组织和胚性愈伤组织酶谱差异明显,胚性愈伤组织中EST和AMY同工酶酶带多且活性高,非胚性愈伤组织中缺乏EST和AMY同工酶表达,AMY同工酶可作为胚性细胞分化和发育的重要标志。香果树体细胞胚形态建成过程中,球形胚时期的AMY、POD、EST同工酶活性最强,表明这一时期生理代谢旺盛,是体细胞胚形态建成的关键时期;POD、AMY和SOD3种同工酶的酶谱及表达强弱在形态建成的不同时期呈现有规律的变化,可作为香果树体细胞胚发生发育特定时期的参考标记。Non-denaturing polyacrylamide gel electrophoresis was used as a tool to analyze isozyme patterns such as peroxidase （POD）, esterase （EST）, amylase （AMY） and superoxide dismutase （SOD） in somatic embryogenesis of Ernmenopterys henryi Oliv.. The results showed that the activity variations of POD, EST, AMY and SOD were closely related to the induction of embryogenic callus and the development of so- matic embryos in somatic embryogenesis of E. henryi. The isozyme analysis showed apparent differences between non-embryogenic calluses and embryogenie calluses. There was a high expression of EST and AMY isozymes in embryogenic calluses, but no in non-embryogenic calli, and the expression of AMY isozyme could act as an important markers. A higher expression of AMY,POD and EST isoenzymes indica- ted that there was a higher physiological metabolic rate in the stage of globular embryos,and this develop- mental stage is the key stage during the morphogenesis of somatic embryos of E. henryi. The results on isozyme patterns during the morphogenesis of somatic embryos of E. henryi showed that the regular varia- tions in increase or decline of the activities of POD, EST,AMY and SOD isozymes were presence with the developmental of somatic embryos,and would be as a reference label of the specific developmental stages.

关 键 词：香果树 体细胞胚胎发生 同工酶 聚丙烯酰胺凝胶电泳 

分 类 号：Q945.6[生物学—植物学]