人巨细胞病毒感染与系统性红斑狼疮病情活动性的关联性研究  

Association between human cytomegalovirus infection and systemlupus erythematosus disease activity index

作　　者：崔寰[1] 陈伟[1] 夏俊保[1] 刘倩[1] 黄维[1] 李迎伟[2] 王明丽[1] 

机构地区：[1]安徽医科大学微生物学教研室、安徽省重要遗传病基因资源利用重点实验室生物工程部,合肥230032 [2]安徽医科大学第一附属医院风湿科,合肥230022

出　　处：《安徽医科大学学报》2008年第5期508-511,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省生物医药重大科技专项(编号:01303003);安徽省教育厅自然科学基金(编号:2006KJ320B)

摘　　要：目的探索人巨细胞病毒(HCMV)感染与系统性红斑狼疮(SLE)病情活动之间的关系。方法采用病毒分离、聚合酶链式反应(PCR)检测HCMVUL54基因和间接免疫荧光试验(IFA)检测HCMVpp65抗原的方法,对49份SLE患者血白细胞标本进行HCMV分离鉴定,并结合临床资料分析HCMV感染与SLE病情活动的相关性。结果49份SLE患者血白细胞标本中,HCMV病毒分离后出现典型细胞病变的有3份,经PCR和IFA联合检测鉴定阳性的有12份,阳性率为24.5%;Logistic回归分析显示,SLE活动期患者的HCMV感染率为38.5%,显著高于SLE非活动期患者8.7%的HCMV感染率(OR=8.7,95%CI:1.4～53.3,P=0.019)。结论HCMV感染与SLE病情活动呈现显著正相关。Objective To study the association between human cytomegalovirus（HCMV）infection and systemlupus erythematosus disease activity.Methods HCMV isolation,HCMV UL54 gene using polymerase chain reaction（PCR）and HCMV pp65 antigen with indirect immunofluorescence assay（IFA）,were assessed in 49 leucocyte samples of systemic lupus erythematosus（SLE）patients.Results With HCMV isolation,3 typical cytopathic effects（CPE）were found in 49 samples.The PCR combined with IFA was performed,and 12 SLE samples infected by HCMV were found in 49 SLE patients,the rate of infection was 24.5%.The results of Logistic regression model indicated that HCMV infection rate of in active phases（38.5%）was significantly higher than that of those in inactive phases（8.7%）（OR=8.7,95% CI：1.4~53.3,P=0.019）.Conclusion Disease activity of SLE patients is positively associated with HCMV infection.

关 键 词：红斑狼疮 系统性/病毒学 巨细胞病毒/分离和提纯 

分 类 号：R373.9[医药卫生—病原生物学] R392.11[医药卫生—基础医学]